Platelet-derived growth factor exerts disparate effects on odontoblast differentiation depending on the dimers in rat dental pulp cells

Autor: Fujieda K, Satoshi Yokose, Naoto Tajima, Akihiko Hasegawa, Hiroshi Kadokura, Hiroshi Sakagami, Tadashi Katayama
Rok vydání: 2003
Předmět:
medicine.medical_specialty
Histology
Platelet-derived growth factor
medicine.medical_treatment
Sialoglycoproteins
Osteocalcin
Odontoblast differentiation
Pathology and Forensic Medicine
Rats
Sprague-Dawley
chemistry.chemical_compound
stomatognathic system
Internal medicine
medicine
Animals
Humans
Receptors
Platelet-Derived Growth Factor
RNA
Messenger
Protein Precursors
Cells
Cultured
Dental Pulp
Platelet-Derived Growth Factor
Extracellular Matrix Proteins
Lymphokines
biology
Odontoblasts
Growth factor
Cell Differentiation
Cell Biology
Alkaline Phosphatase
Phosphoproteins
Cell biology
Rats
Incisor
stomatognathic diseases
Endocrinology
chemistry
Dentin
biology.protein
Dentinogenesis
Alkaline phosphatase
Dental Pulp Calcification
Calcium
Female
Dimerization
Platelet-derived growth factor receptor
Dentin sialoprotein
Zdroj: Cell and tissue research. 315(3)
ISSN: 0302-766X
Popis: Platelet-derived growth factor (PDGF) has recently been demonstrated to control the expression of alkaline phosphatase and proteoglycan synthesis of odontoblastic cells in dental pulp tissues. Although PDGF appears to be closely related to dentinogenesis, much about the mode of action of PDGF on odontoblast differentiation remains unclear. In this study, we examined the effects of three PDGF dimers (PDGF AA, AB, and BB) on odontoblastic differentiation of dental pulp cells in long-term mineralized cultures. Dental pulp cells isolated from rat lower incisors were continuously treated with each of PDGF AA, AB, and BB in separate cultures for 20 days. The three PDGF dimers suppressed alkaline phosphatase activity, osteocalcin and calcium content, and the formation of dentin-like nodules. The expression of mRNA for dentin sialoprotein (DSP) in the cells was inhibited by PDGF AA treatment, whereas PDGF AB and BB treatment stimulated the expression of DSP, even though the dentin-like nodule formation was inhibited. Although the effects of PDGF on odontoblastic differentiation varied among the dimers, the cells expressed both PDGF alpha and beta receptors, whose quantities were similar. These results suggest that PDGF exerts diverse effects on odontoblastic differentiation depending on its dimeric form. These in vitro findings explain, at least in part, the in vivo action of PDGF in dentinogenesis during the repair process of damaged dental pulp.
Databáze: OpenAIRE
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