Deletion of the glucocorticoid receptor chaperone FKBP51 prevents glucocorticoid-induced skin atrophy
| Autor: | Irina Budunova, Joel T. Dudley, Edwin R. Sanchez, Pankaj Bhalla, Lance A. Stechschulte, Weinian Shou, Ben Readhead, Gleb Baida, Alexander Yemelyanov |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Adipose tissue News 03 medical and health sciences 0302 clinical medicine Glucocorticoid receptor Dermis Internal medicine glucocorticoid receptor medicine Protein kinase B PI3K/AKT/mTOR pathway integumentary system Chemistry Akt 3. Good health HaCaT FKBP51 030104 developmental biology medicine.anatomical_structure Endocrinology Oncology 030220 oncology & carcinogenesis Phosphorylation glucocorticoid skin atrophy Glucocorticoid Research Paper medicine.drug |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| Popis: | FKBP51 (FK506-binding protein 51) is a known co-chaperone and regulator of the glucocorticoid receptor (GR), which usually attenuates its activity. FKBP51 is one of the major GR target genes in skin, but its role in clinical effects of glucocorticoids is not known. Here, we used FKBP51 knockout (KO) mice to determine FKBP51's role in the major adverse effect of topical glucocorticoids, skin atrophy. Unexpectedly, we found that all skin compartments (epidermis, dermis, dermal adipose and CD34+ stem cells) in FKBP51 KO animals were much more resistant to glucocorticoid-induced hypoplasia. Furthermore, despite the absence of inhibitory FKBP51, the basal level of expression and glucocorticoid activation of GR target genes were not increased in FKBP51 KO skin or CRISPR/Cas9-edited FKBP51 KO HaCaT human keratinocytes. FKBP51 is known to negatively regulate Akt and mTOR. We found a significant increase in AktSer473 and mTORSer2448 phosphorylation and downstream pro-growth signaling in FKBP51-deficient keratinocytes in vivo and in vitro. As Akt/mTOR-GR crosstalk is usually negative in skin, our results suggest that Akt/mTOR activation could be responsible for the lack of increased GR function and resistance of FKBP51 KO mice to the steroid-induced skin atrophy. |
| Databáze: | OpenAIRE |
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