Papain immobilization on heterofunctional membrane bacterial cellulose as a potential strategy for the debridement of skin wounds
| Autor: | Arcelina Pacheco Cunha, Niédja Fittipaldi Vasconcelos, Maria de Fátima Borges, Lídia de Araújo Pinto Vieira, Nágila M.P.S. Ricardo, Fábia Karine Andrade, Ana Iraidy S. Brígida, Rodrigo Silveira Vieira, Morsyleide de Freitas Rosa, Rosemayre Souza Freire |
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| Rok vydání: | 2020 |
| Předmět: |
Chemical structure
02 engineering and technology Biochemistry 03 medical and health sciences chemistry.chemical_compound Adsorption Structural Biology Papain Cellulose Oxidized Molecular Biology Skin 030304 developmental biology 0303 health sciences Chromatography Ion exchange General Medicine Hydrogen-Ion Concentration Enzymes Immobilized 021001 nanoscience & nanotechnology Membrane chemistry Covalent bond Bacterial cellulose Acetobacteraceae Fermentation 0210 nano-technology Oxidation-Reduction Peptide Hydrolases |
| Zdroj: | International Journal of Biological Macromolecules. 165:3065-3077 |
| ISSN: | 0141-8130 |
| Popis: | We combined the chemical and physical methods of papain immobilization through the aldehyde groups available on oxidized bacterial cellulose (OxBC) to provide high proteolytic activity for future applications as bioactive dressing. Bacterial cellulose (BC) was obtained by the fermentation of Komagataeibacter hansenii in Hestrin-Schramm medium for 5 days, followed by purification and oxidation using NaIO4. Surface response methodology was used to optimize papain immobilization (2%, w/v) for 24 h. The independent variables: pH (3–7) and temperature (5 to 45 °C) were investigated. The mathematically validated optimal conditions of 45 °C and pH 7 had a statistical effect on the immobilization yield (IY) of papain in OxBC (52.9%). These ideal conditions were also used for papain immobilization in BC (unoxidized). The IY of 9.1% was lower than that of OxBC. OxBC-Papain and BC-Papain were investigated using thermal analysis, confocal microscopy, and diffusion testing. The OxBC support exhibited a more interactive chemical structure than the BC support, and was capable of immobilizing papain by covalent bonds (-C-NHR) and adsorption (ion exchange), with 93.3% recovered activity, 49.4% immobilization efficiency, and better thermal stability. Papain immobilized to OxBC by adsorption displayed 53% widespread papain activity. The results indicate the potential of prolonged bioactivity in debrided chronic wounds. |
| Databáze: | OpenAIRE |
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