The fluorescent detection of Paradiplozoon sp. (Monogenea: Diplozoidae) attachment clamps' sclerites and integumental proteins : research communication

Autor:	S. J. Milne, Annemariè Avenant-Oldewage
Rok vydání:	2006
Předmět:	lcsh:Veterinary medicine General Veterinary biology General Medicine Anatomy biology.organism_classification Fluorescence Molecular biology Staining chemistry.chemical_compound Light source chemistry Ultrastructure lcsh:SF600-1100 Fluorescein Monogenea
Zdroj:	Onderstepoort Journal of Veterinary Research, Vol 73, Iss 2, Pp 149-152 (2006)
ISSN:	2219-0635 0030-2465
DOI:	10.4102/ojvr.v73i2.161
Popis:	MILNE, S.J. & AVENANT-OLDEWAGE, A. 2006. The fluorescent detection of Paradiplozoon sp. (Monogenea: Diplozoidae) attachment clamps’ sclerites and integumental proteins. Onderstepoort Jour nal of Veterinary Research, 73:149–152 In the genus Paradiplozoon, few hard structures are present therefore most of the taxonomic studies have focused on the unique ultrastructure of the sclerites. Alcohol-fixed specimens were transferred to BSA-saline for 5 min. before staining indefinitely with 5 μl WGA-TRX, 5 μl SYTO 9 and 5 μl of Cal co fluor White M2R. Rhodamine, Fluorescein and DAPI bandpass filters on the microscope enabled selective light wavelength illumination of the three flourochromes by a mercury light source. This method provided an easy and rapid methodology to show the internal sclerites of attachment clamps. It is suitable for alcohol preserved specimens and may have additional applications in other helminth organisms.
Databáze:	OpenAIRE
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