Cancerous and non-neoplastic stem cells in the stomach similarly express CD44 and CD133
| Autor: | Paulo Roberto Carvalho Almeida, Neli Patrícia Pereira Feitosa, Venúcia Bruna Magalhães Pereira, Deysi Viviana Tenazoa Wong, Carlos Henrique Alencar, Andréia Victoria Franklin Queroz, Marcelo Leite Vieira Costa, Cristiane Cunha Frota, Roberto C. P. Lima-Júnior, Bruno Gadelha Bezerra Silva, Bruno Jucá Rodrigues |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Pathology medicine.medical_specialty Histology Adenocarcinoma Stem cell marker Cancer stem cell Stomach Neoplasms medicine Gastric mucosa Humans AC133 Antigen Lymph node Aged business.industry Stomach Cancer Intestinal metaplasia Cell Biology General Medicine Middle Aged medicine.disease Immunohistochemistry digestive system diseases Neoplasm Proteins medicine.anatomical_structure Hyaluronan Receptors Gastric Mucosa Cancer cell Neoplastic Stem Cells business |
| Zdroj: | Acta histochemica. 123(7) |
| ISSN: | 1618-0372 |
| Popis: | CD44 and CD133 have been considered as cancer stem cell (CSC) markers. Stem cell markers are rarely described in healthy stomach tissues. However, the clinicopathological and prognostic value of CD44 and CD133 in gastric cancer remains controversial. This study investigated the expression of CD44 and CD133 in gastric cancer and non-neoplastic gastric mucosa. We used samples of primary gastric adenocarcinomas (n = 69), metastatic lymph nodes (n = 30), intestinal metaplasia (n = 17), and histologically normal gastric tissues of surgical margins (n = 54). The expression of CD44 and CD133 were studied in samples by immunohistochemistry. Fisher’s exact test and a logistic regression model were used in this study. CD44 expression was observed in 12% of samples with intestinal metaplasia, 20% with lymph node metastases, 22% with normal mucosa, to 30% of samples with primary tumors. Most of these positive tumors showed immunostaining in less than 4% of cancerous cells, mainly in the diffuse type. CD133 expression was observed in 7% (intestinal metaplasia) to 46% (normal mucosa). In the positive cases of cancer (24%), in most of them, less than 3% of cells were marked. CD44 and CD133 expression in the histologically normal gastric mucosa was restricted to the deeper regions of the gastric crypts at the level where stem cells and progenitor cells are usually found. CD44 and CD133 expression occurs in few gastric cancer cells, mainly in diffuse carcinomas, and are expressed in histologically normal gastric mucosae. None of the markers are specific for cancer and are also present in intestinal metaplasia and the normal mucosa. |
| Databáze: | OpenAIRE |
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