Inhibition of Adenylate Cyclase of Catfish and Rat Hepatocyte Membranes BY 9-(Tetrahydro-2-Furyl)Adenine (SQ 22536)
| Autor: | C. Ottolenghi, L. Brighenti, Elena Fabbri |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Male
medicine.medical_specialty Epinephrine GTP' Adenylate kinase Biology Biochemistry Glucagon Cyclase Norepinephrine Phenylephrine Species Specificity Internal medicine medicine Animals heterocyclic compounds Cells Cultured Adenine Cell Membrane Isoproterenol Adenosine Rats Ictaluridae Kinetics medicine.anatomical_structure Endocrinology Liver Hepatocyte Adenylyl Cyclase Inhibitors Sodium Fluoride Molecular Medicine Female Guanosine Triphosphate Cyclase activity Catfish medicine.drug |
| Zdroj: | Journal of Enzyme Inhibition. 5:87-98 |
| ISSN: | 8755-5093 |
| DOI: | 10.3109/14756369109069062 |
| Popis: | The adenosine analogue 9-(Tetrahydro-2-furyl)adenine, SQ 22536, inhibited adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] activity of crude membrane preparations from catfish (Ictalurus melas) and rat isolated hepatocytes in a non-competitive manner. The IC50s were reduced in the presence of NaF. SQ 22536 reduced the activity of adenylate cyclase also in the presence of increasing concentrations of GTP, as well as Mg++ and Mn++. In the presence of catecholamines (epinephrine, norepinephrine, isoproterenol, phenylephrine) SQ 22536 reduced their activating effect on adenylate cyclase in both catfish and rat membranes. SQ 22536 also inhibited the effect of glucagon (0.1 UM) on rat membrane cyclase activity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|