Unlocking a caged lysosomal protein from a polymeric nanogel with a pH trigger
| Autor: | Derrick T Deming, Priyaa Prasad, Sankaran Thayumanavan, Tyler M. Marcinko, Mijanur Rahaman Molla, Scott C. Garman |
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| Rok vydání: | 2014 |
| Předmět: |
chemistry.chemical_classification
Polymers and Plastics Protein molecules Chemistry Polymers Therapeutic treatment Nanogels Proteins Bioengineering Hydrogen-Ion Concentration Article Polyethylene Glycols Biomaterials Enzyme HEK293 Cells Biochemistry Turn off Cell Line Tumor Materials Chemistry Humans Polyethyleneimine Nanogel |
| Zdroj: | Biomacromolecules |
| ISSN: | 1526-4602 |
| Popis: | A polymeric nanogel has been used to sequester and turn off a lysosomal protein, acid α-glucosidase (GAA). The nanogel contains a β-thiopropionate cross-linker, which endows the nanogel with pH-sensitivity. While encapsulation of the enzyme fully turns off its activity, approximately 75% of the activity is recovered upon reducing the pH to 5.0. The recovered activity is ascribed to pH-induced degradation of the β-thiopropionate cross-linker causing the swelling of the nanogel and ultimately causing the release of the enzyme. We envision that strategies for sequestering protein molecules and releasing them at lysosomal pH might open up new directions for therapeutic treatment of lysosomal storage diseases. |
| Databáze: | OpenAIRE |
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