Nonsteroidal anti-inflammatory drugs act as endocrine disruptors in Astyanax lacustris (Teleostei: Characidae) reproduction: An ex vivo approach

Autor: Giovana Souza Branco, Renata Guimarães Moreira, Marília de Paiva Camargo, Maria Inês Borella, Carlos Eduardo Tolussi, Marcela Muñoz-Peñuela, Aline Dal’Olio Gomes
Přispěvatelé: Universidade Estadual Paulista (Unesp), Universidade de São Paulo (USP), Universidade Anhembi Morumbi – R. Dr. Almeida Lima
Rok vydání: 2021
Předmět:
Zdroj: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 0166-445X
DOI: 10.1016/j.aquatox.2021.105767
Popis: Made available in DSpace on 2021-06-25T10:51:38Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-03-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Pharmaceutical products can act as endocrine-disrupting compounds (EDCs), affecting the physiological processes of animals, such as development or reproduction. This study aimed to investigate the influence of different concentrations of nonsteroidal anti-inflammatory drugs (NSAIDs) diclofenac (DCF) and ibuprofen (IBU) alone and mixed (MIX) on gonadotropin gene expression and gonadal steroid release using Astyanax lacustris pituitary and testes explant systems, respectively. The explant organs were maintained for 12 h in Leibovitz (L-15) medium supplemented with 0, 0.1, 1, 10, 100, and 1000 ng L−1 of DCF, IBU, and MIX (ratio 1:1 of the same concentrations of DCF and IBU alone) and gonadotropin releasing-hormone (cGnRH2) stimulation in pituitary explants and human chorionic gonadotropin (hCG) stimulation in testes explants. The pituitary glands and the media from the testicular explants were collected for gene expression analysis including the β subunit of the follicle-stimulating hormone (fshβ) and luteinizing hormone (lhβ) and secreted gonadal steroid concentration analysis, respectively. Both DCF and IBU (alone and mixed) decreased pituitary gene expression of fshβ and lhβ and this inhibitory effect was evident even at low concentrations. In the testes, DCF and IBU did not change the levels of estradiol, and both pharmaceuticals increased the release of 11-ketotestosterone at low doses, while only IBU decreased the levels of testosterone in all concentrations. IBU's inhibitory effect in the testes was not triggered by the mixture of the two drugs. These results suggest that NSAIDs, may interfere in fish reproduction by acting as EDCs, thereby negatively affecting A. lacustris spermatogenesis and maturation. Centro de Aquicultura da Universidade Estadual Paulista (CAUNESP) Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), V. Acesso Prof. Paulo Donato Castelane s/n Laboratório de Metabolismo e Reprodução de Organismos Aquáticos Departamento de Fisiologia Instituto de Biociências Universidade de São Paulo Rua do Matão, Trav.14, nº 321 Laboratório de Endocrinologia de Peixes Departamento de Biologia Celular e do Desenvolvimento Instituto de Ciências Biomédicas Universidade de São Paulo, Av. Prof. Lineu Prestes n. 1524, lab 426 Escola de Ciências da Saúde Universidade Anhembi Morumbi – R. Dr. Almeida Lima, 1134 - Parque da Mooca Centro de Aquicultura da Universidade Estadual Paulista (CAUNESP) Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), V. Acesso Prof. Paulo Donato Castelane s/n FAPESP: 2014/16320-7
Databáze: OpenAIRE
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