Molecular characterization of a Trichinella spiralis serine proteinase

Autor: Jing Cui, Ruo Dan Liu, Fang Liu, Chen Xi Hu, Xiang Yuan Sun, Ying Bai, Xi Zhang, Xin Yue, Zhong Quan Wang, Qi Da Yang
Přispěvatelé: Zhengzhou University
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
030231 tropical medicine
Trichinella spiralis
Spleen
immunogenicity
Immunoglobulin E
Microbiology
03 medical and health sciences
Mice
0302 clinical medicine
Immune system
serine proteinase
parasitic diseases
medicine
Animals
[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology
Amino Acid Sequence
Phylogeny
Antibody-dependent cell-mediated cytotoxicity
Immunity
Cellular
Mice
Inbred BALB C
[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health
lcsh:Veterinary medicine
General Veterinary
biology
Immunogenicity
fungi
intestinal epithelial cells (IECs)
Helminth Proteins
biology.organism_classification
invasion
3. Good health
Immunity
Humoral
030104 developmental biology
medicine.anatomical_structure
Secretory protein
biology.protein
lcsh:SF600-1100
Female
[SDV.IMM.VAC]Life Sciences [q-bio]/Immunology/Vaccinology
Antibody
Serine Proteases
ADCC
Sequence Alignment
Research Article
Zdroj: Veterinary Research, Vol 51, Iss 1, Pp 1-19 (2020)
Veterinary Research
Veterinary Research, BioMed Central, 2020, 51 (1), pp.125. ⟨10.1186/s13567-020-00847-0⟩
ISSN: 1297-9716
0928-4249
DOI: 10.1186/s13567-020-00847-0
Popis: The aim of this study was to investigate the biological characteristics and functions of a Trichinella spiralis serine proteinase (TsSerp) during larval invasion and development in the host. The full-length TsSerp cDNA sequence was cloned and expressed in Escherichia coli BL21. The results of RT-PCR, IFA and western blotting analyses showed that TsSerp was a secretory protein that was highly expressed at the T. spiralis intestinal infective larva and muscle larva stages and primarily located at the cuticle, stichosome and intrauterine embryos of the parasite. rTsSerp promoted the larval invasion of intestinal epithelial cells (IECs) and the enteric mucosa, whereas an anti-rTsSerp antibody impeded larval invasion; the promotion and obstruction roles were dose-dependently related to rTsSerp and the anti-rTsSerp antibodies, respectively. Vaccination of mice with rTsSerp elicited a remarkable humoral immune response (high levels of serum IgG, IgG1/IgG2a, IgE and IgM), and it also triggered both systemic (spleen) and local intestinal mucosal mesenteric lymph node (MLN) cellular immune responses, as demonstrated by a significant elevation in Th1 cytokines (IFN-γ) and Th2 cytokines (IL-4) after the spleen and MLN cells from vaccinated mice were stimulated with rTsSerp. Anti-TsSerp antibodies participated in the killing and destruction of newborn larvae via ADCC. The mice vaccinated with rTsSerp exhibited a 48.7% reduction in intestinal adult worms and a 52.5% reduction in muscle larvae. These results indicated that TsSerp participates in T. spiralis invasion and development in the host and might be considered a potential candidate target antigen to develop oral polyvalent preventive vaccines against Trichinella infection.
Databáze: OpenAIRE
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