Methylation of RUNX3 and RASSF1A and the risk of Malignancy in small solitary pulmonary nodules

Autor:	Cui Xiangyu, Zhao Jinglan, Lu Ruizhen, Zhang Zhixue, Peng Chen, Huang Xiuying
Rok vydání:	2019
Předmět:	Adult Male 0301 basic medicine endocrine system Lung Neoplasms Biology RUNX3 gene lcsh:RC254-282 law.invention 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Western blot law Biopsy Biomarkers Tumor medicine Humans Radiology Nuclear Medicine and imaging RASSF1A gene Promoter Regions Genetic Polymerase chain reaction Aged Aged 80 and over Solitary pulmonary nodule DNA methylation medicine.diagnostic_test Tumor Suppressor Proteins Solitary Pulmonary Nodule Promoter small solitary pulmonary nodule General Medicine Methylation Middle Aged Prognosis lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens medicine.disease Molecular biology digestive system diseases Gene Expression Regulation Neoplastic Core Binding Factor Alpha 3 Subunit 030104 developmental biology Oncology chemistry 030220 oncology & carcinogenesis Female DNA Follow-Up Studies
Zdroj:	Journal of Cancer Research and Therapeutics, Vol 15, Iss 4, Pp 899-903 (2019)
ISSN:	0973-1482
DOI:	10.4103/jcrt.jcrt_790_18
Popis:	Background: This study aimed to evaluate the methylation of RUNX3 and RASSF1A gene promoter regions as a marker to distinguish between benign and malignant of small solitary pulmonary nodule (SPN) ≤10 mm in size. Materials and Methods: A total of 147 patients with pathologically confirmed SPNs were enrolled. DNA samples were extracted from biopsy tissues or serum. Methylation of RUNX3 and RASSF1A gene promoter regions was detected by the methylation-specific polymerase chain reaction. The expression of RUNX3 and RASSF1A in SPN tissues was detected by western blot. Results: Of the 147 patients, 89 had benign SPNs and 58 had malignant SPNs. The rate of serum RUNX3 and RASSF1A gene methylation in malignant SPNs was significantly higher than that in benign SPNs (65.5% vs. 12.3%, and 67.2% vs. 10.1%, respectively; P < 0.05). The expression of RUNX3 and RASSF1A in malignant SPN tissues was lower than that in benign SPN tissues. The hypermethylation status of RUNX3 or RASSF1A genes was not significantly associated with age, gender, and smoking. Conclusions: The methylation level of the RUNX3 and RASSF1A gene promoter regions is a promising marker for assessing SPNs.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::646d7bec0eda2ca904aef93aeb39a2ce https://doi.org/10.4103/jcrt.jcrt_790_18 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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