Measurement of peripheral B cell subpopulations in common variable immunodeficiency (CVID) using a whole blood method
Autor: | Michael Schlesier, John R Jones, E. Bateman, N. Woodham, Klaus Warnatz, S. A. Misbah, Hans-Hartmut Peter, Helen Chapel, Berne Ferry |
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Rok vydání: | 2005 |
Předmět: |
Adult
Male Pathology medicine.medical_specialty Adolescent Immunology Peripheral blood mononuclear cell Immunopathology Clinical Studies medicine Humans Immunology and Allergy Lymphocyte Count Immunodeficiency B cell Aged Whole blood B-Lymphocytes biology Common variable immunodeficiency Reproducibility of Results Middle Aged Flow Cytometry medicine.disease In vitro Tumor Necrosis Factor Receptor Superfamily Member 7 Common Variable Immunodeficiency medicine.anatomical_structure Leukocytes Mononuclear biology.protein Female Receptors Complement 3d Antibody Immunologic Memory |
Zdroj: | Clinical and Experimental Immunology. 140:532-539 |
ISSN: | 1365-2249 0009-9104 |
DOI: | 10.1111/j.1365-2249.2005.02793.x |
Popis: | Summary Recent reports have described reduced populations of CD27+ memory B cells and increased percentages of undifferentiated B cells in peripheral blood of patients with common variable immunodeficiency (CVID). This work has prompted two attempts to classify CVID based on rapid flow cytometric quantification of peripheral blood memory B cells and immature B cells. Evidence to support the hypothesis that such in vitro B cell classification systems correlate with clinical subtypes of CVID is being sought. For the classification to be useful in routine diagnosis, it is important that the flow cytometric method can be used without prior separation of peripheral blood mononuclear cells (PBMC). We have examined 23 CVID patients and 24 controls, using both PBMC and whole blood, and find an excellent correlation between these methods. The reproducibility of the method was excellent. We classified the CVID patients by all three of the existing classifications, including secretion of immunoglobulin by B cells in vitro as described by Bryant, as well as the more recent flow cytometric classification methods. Only one patient changed classification as a result of using whole blood. |
Databáze: | OpenAIRE |
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