Using UPLC-MS/MS for Characterization of Active Components in Extracts of Yupingfeng and Application to a Comparative Pharmacokinetic Study in Rat Plasma after Oral Administration

Autor: Mengqi Jia, Ye-Juan Xiong, Yun Xue, Yan Wang, Chao Yan
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Male
Pharmaceutical Science
Administration
Oral
chemical constituents
Tandem mass spectrometry
Mass spectrometry
01 natural sciences
Article
Analytical Chemistry
Rats
Sprague-Dawley
lcsh:QD241-441
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Astragaloside
Pharmacokinetics
lcsh:Organic chemistry
Coumarins
Polysaccharides
Drug Discovery
tandem mass spectrometry
Formononetin
Animals
liquid chromatography
Physical and Theoretical Chemistry
Medicine
Chinese Traditional
Medicinal plants
integrated pharmacokinetics
Chromatography
High Pressure Liquid
Flavonoids
Chromatography
Plant Extracts
Yupingfeng formula
010401 analytical chemistry
Organic Chemistry
Saponins
0104 chemical sciences
Rats
Calycosin
chemistry
Chemistry (miscellaneous)
Chromones
030220 oncology & carcinogenesis
Chromone
Molecular Medicine
Drugs
Chinese Herbal
Zdroj: Molecules; Volume 22; Issue 5; Pages: 810
Molecules, Vol 22, Iss 5, p 810 (2017)
Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry
Molecules
ISSN: 1420-3049
DOI: 10.3390/molecules22050810
Popis: Yupingfeng (YPF), a famous traditional Chinese medicine, which contains a large array of compounds, has been effectually used in health protection. A two-dimensional liquid chromatography (²D-LC) combined with quadrupole time-of-flight mass spectrometry (QTOF-MS) method was firstly established to separate and identify chemical components in YPF. A total of 33 compounds were identified, including 15 constituents (flavonoids and saponins) in Astragali radix; seven constituents (sesquiterpenoids and polysaccharide) in Atractylodis rhizoma; and 11 constituents (chromone and coumarins) in Saposhnikoviae radix. The corresponding fragmentation pathway of typical substances was investigated. Then, seven active constituents (astragaloside, calycosin, formononetin, cimicifugoside, 4-O-beta-d-glucosyl-5-O-methylvisamminol, sec-O-glucosylhamaudol, and atractylenolide II) derived from three medicinal plants were chosen to further investigate the pharmacokinetic behavior of YPF formula using ultrahigh-performance liquid chromatography with triple quadrupole mass spectrometry system. The method was sensitive, accurate and reliable. We also used the area under the plasma concentration-time curve from zero to infinity (AUC0-∞) as weighting factor to make an integrated pharmacokinetic curve. Results show that the constituents of Saposhnikoviae radix have the best absorption and pharmacokinetic behavior and may play important role in leading to the changes of overall therapeutic effects of YPF. Further study is needed to confirm the association between them.
Databáze: OpenAIRE
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