Differential Effects of LPS and 6-OHDA on Microglia's Morphology in Rats: Implications for Inflammatory Model of Parkinson's Disease
| Autor: | Liliana Mendieta, Isabel Rodríguez Martínez, Gabriel Ramirez-Garcia, Yousef Tizabi, Irving Parra |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Lipopolysaccharides Male medicine.medical_specialty Parkinson's disease Time Factors Microinjections Tyrosine 3-Monooxygenase Substantia nigra Inflammation Motor Activity Toxicology 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Animals Parkinson Disease Secondary Oxidopamine Pars Compacta Tyrosine hydroxylase Microglia Pars compacta business.industry General Neuroscience Dopaminergic Neurons Dopaminergic Neurodegeneration medicine.disease Corpus Striatum Rats Disease Models Animal 030104 developmental biology Endocrinology medicine.anatomical_structure nervous system medicine.symptom business 030217 neurology & neurosurgery |
| Zdroj: | Neurotoxicity research. 37(1) |
| ISSN: | 1476-3524 |
| Popis: | Parkinson’s disease (PD) is an idiopathic and progressive neurodegenerative disease characterized by the loss of ~ 80% of dopaminergic neurons in substantia nigra pars compacta (SNpc). Because activation of the innate cellular immune response, mediated by microglia, has been linked to the neurodegeneration in PD, in the present study, we evaluated the effects of lipopolysaccharide (LPS) and 6-hydroxydopamine (6-OHDA) on microglia’s morphology, reflective of their activity, as well as tyrosine hydroxylase (TH)-positive neurons in SNpc and motor behavior. Adult male Wistar rats were stereotactically injected with LPS or 6-OHDA into the left dorsolateral striatum. Control groups received appropriate vehicle. The morphological changes of microglial cells and neurotoxic effects were examined at 1, 7, and 14 post-injection days. Both LPS and 6-OHDA caused activation and morphological changes in microglial cells as well as loss of dopaminergic neurons in SNpc. These effects were maximal at 14 days post-injection where motor impairments were also evident. However, our findings indicate that 6-OHDA causes a low degree of microglia activation compared to LPS. Hence, it may be concluded that LPS model of PD might be a better representation of inflammatory involvement in this devastating disease. |
| Databáze: | OpenAIRE |
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