Extension of the four-parameter logistic model for ELISA to multianalyte analysis
| Autor: | Bruce D. Hammock, Monika Wortberg, Shirley J. Gee, Sabine B. Kreissig, David M. Rocke, David S. Bunch, Geoffrey P. Jones |
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| Rok vydání: | 1994 |
| Předmět: |
Analyte
medicine.diagnostic_test Chemistry Triazines Immunology Analytical chemistry Dose-Response Relationship Immunologic Cross reactions Enzyme-Linked Immunosorbent Assay Extension (predicate logic) Elisa assay Cross Reactions Logistic regression Extended model Immunoassay Statistics medicine Immunology and Allergy Logistic function |
| Zdroj: | Journal of immunological methods. 177(1-2) |
| ISSN: | 0022-1759 |
| Popis: | The standard implementation of enzyme-linked immunosorbent assay (ELISA) for single analytes can lead to false conclusions if cross reacting compounds are present in the sample. This paper discusses the extension of the usual four-parameter logistic model for ELISA to the case of multiple cross-reacting analytes. The use of the extended model in multianalyte analysis (MELISA) is illustrated and compared with a more simplistic approach. Data on the analysis of a binary mixture of s-triazines suggests the superiority of the proposed model. This model is also suitable for other forms of immunoassay that use the four-parameter logistic curve. |
| Databáze: | OpenAIRE |
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