Determination of protamine sulphate in drug formulations using high performance liquid chromatography
| Autor: | A Snycerski, Jadwiga Tautt, Jadwiga Dudkiewicz-Wilczyńska |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Chromatography
Protamine sulfate Clinical Biochemistry Pharmaceutical Science Heparin Antagonists Reproducibility of Results High-performance liquid chromatography Drug formulations Dosage form Analytical Chemistry chemistry.chemical_compound Protamine sulphate chemistry Pharmaceutical Preparations Drug Discovery Trifluoroacetic acid medicine Protamines Acetonitrile Quantitative analysis (chemistry) Spectroscopy Chromatography High Pressure Liquid medicine.drug |
| Zdroj: | Journal of pharmaceutical and biomedical analysis. 18(4-5) |
| ISSN: | 0731-7085 |
| Popis: | Protamine sulphate, which has the property of neutralising heparin, is determined in pharmaceutical formulations using spectrophotometric (BP 1995) or biochemical methods (USP XXIII 1995). Accuracy of these methods is not very high. We applied the HPLC technique for the assay of protamine sulphate in a gel formulation. The assay was carried out on a diol-type column with a mobile phase containing 8% acetonitrile in 0.15% trifluoroacetic acid at pH 2.5. The flow rate was 1.0 ml min(-1). The results obtained show that HPLC can be used for the determination of protamine sulphate in pharmaceutical preparations. The method is rapid and more accurate than those described until now. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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