LC–MS/MS Analysis of Thiol-Containing Amino Acids in Exosomal Fraction of Serum
| Autor: | Hideaki Ichiba, Mayu Onozato, Takeshi Fukushima, Kana Kobata, Tatsuya Sakamoto |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Homocysteine
Exosomes Mass spectrometry medicine.disease_cause 01 natural sciences Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Tandem Mass Spectrometry medicine Humans Cysteine 030304 developmental biology chemistry.chemical_classification 0303 health sciences Chromatography Chemistry 010401 analytical chemistry Reproducibility of Results Dipeptides General Medicine Glutathione 0104 chemical sciences Amino acid Thiol Composition (visual arts) Oxidative stress Chromatography Liquid |
| Zdroj: | Journal of Chromatographic Science. 58:636-640 |
| ISSN: | 1945-239X 0021-9665 |
| DOI: | 10.1093/chromsci/bmaa028 |
| Popis: | It has been suggested that thiol-containing amino acids could be used as biomarkers for diseases associated with oxidative stress. We investigated the thiol-containing amino acids, homocysteine (Hcy), cysteine (Cys), glutathione (GSH) and γ-glutamylcysteine (γ-GluCys), in commercial human serum by using liquid chromatography–tandem mass spectrometry (LC–MS/MS) after precolumn derivatization with 4-fluoro-7-sulfobenzofurazan. This method was applied to determine the composition of thiol-containing amino acids in exosomes prepared from the serum. Hcy, Cys, GSH and γ-GluCys could be detected in the exosomal fraction, and the ratio of each thiol-containing amino acid was similar to those in the corresponding native serum. Cys (94.76%) was most enriched in the exosomal fraction, followed by GSH (2.97%), γ-GluCys (1.59%) and Hcy (0.68%). These findings suggest that thiol-containing amino acids, Hcy, Cys, GSH and γ-GluCys, are included in exosomes in human serum. |
| Databáze: | OpenAIRE |
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