PhenoChip: A single-cell phenomic platform for high-throughput photophysiological analyses of microalgae
| Autor: | Roman Stocker, Michael Kühl, Kang Soo Lee, Lars Behrendt, Vicente I. Fernandez, M. Mehdi Salek, Erik Trampe |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Cyanobacteria Microorganism Photosynthesis Microbiology 01 natural sciences 03 medical and health sciences Symbiodinium chemistry.chemical_compound Transgenerational epigenetics Botany Microalgae Animals Ecotoxicology Stress resilience Phenomics Symbiosis Applied Ecology Research Articles Ecosystem 030304 developmental biology 0303 health sciences Multidisciplinary biology 010604 marine biology & hydrobiology Biochemistry and Molecular Biology SciAdv r-articles Anthozoa biology.organism_classification chemistry Chlorophyll Biokemi och molekylärbiologi Research Article |
| Zdroj: | Science Advances, 6 (36) Science Advances Behrendt, L, Mehdi Salek, M, Trampe, E L, Fernandez, V I, Lee, K S, Kühl, M & Stocker, R 2020, ' PhenoChip : A single-cell phenomic platform for high-throughput photophysiological analyses of microalgae ', Science Advances, vol. 6, no. 36, eabb2754 . https://doi.org/10.1126/sciadv.abb2754 |
| ISSN: | 2375-2548 |
| Popis: | Photosynthetic microorganisms are key players in aquatic ecosystems with strong potential for bioenergy production, yet their systematic selection at the single-cell level for improved productivity or stress resilience (“phenotyping”) has remained largely inaccessible. To facilitate the phenotyping of microalgae and cyanobacteria, we developed “PhenoChip,” a platform for the multiparametric photophysiological characterization and selection of unicellular phenotypes under user-controlled physicochemical conditions. We used PhenoChip to expose single cells of the coral symbiont Symbiodinium to thermal and chemical treatments and monitor single-cell photophysiology via chlorophyll fluorometry. This revealed strain-specific thermal sensitivity thresholds and distinct pH optima for photosynthetic performance, and permitted the identification of single cells with elevated resilience toward rising temperature. Optical expulsion technology was used to collect single cells from PhenoChip, and their propagation revealed indications of transgenerational preservation of photosynthetic phenotypes. PhenoChip represents a versatile platform for the phenotyping of photosynthetic unicells relevant to biotechnology, ecotoxicology, and assisted evolution. Science Advances, 6 (36) ISSN:2375-2548 |
| Databáze: | OpenAIRE |
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