Developmental patterns of protein expression in photoreceptors implicate distinct environmental versus cell-intrinsic mechanisms
| Autor: | P.T. Johnson, Benjamin E. Reese, R.R. Williams |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Opsin
genetic structures G-Protein-Coupled Receptor Kinase 1 Physiology Lipoproteins Peripherins Nerve Tissue Proteins Phosducin GTP-Binding Protein Regulators Intermediate Filament Proteins 3' 5'-Cyclic-GMP Phosphodiesterases Pregnancy Recoverin Hippocalcin Arrestin Animals RNA Messenger Transducin Eye Proteins DNA Primers Membrane Glycoproteins biology Reverse Transcriptase Polymerase Chain Reaction Calcium-Binding Proteins Ferrets Rod Opsins Gene Expression Regulation Developmental Peripherin Phosphoproteins Rod Cell Outer Segment eye diseases Sensory Systems Cell biology Rhodopsin kinase Biochemistry Rhodopsin biology.protein Female sense organs Protein Kinases |
| Zdroj: | Visual Neuroscience. 18:157-168 |
| ISSN: | 1469-8714 0952-5238 |
| DOI: | 10.1017/s0952523801181150 |
| Popis: | The present study has examined the spatial and temporal expression patterns of various proteins associated with the structure and function of mature photoreceptor outer segments in the developing ferret's retina using immunocytochemistry and RT-PCR. One set of proteins, including rod opsin, arrestin, and recoverin, was detected progressively in photoreceptors as they became postmitotic, being expressed well before the differentiation of outer segments. A second set of proteins, including β- and γ-transducin, cGMP-phosphodiesterase, phosducin, rhodopsin kinase, rod cGMP-gated cation channel protein, and peripherin, displayed a contrasting temporal onset and pattern of spatial emergence. These latter proteins first became detectable either shortly before or coincident with outer segment formation, and were expressed simultaneously in both older and younger photoreceptor cells. A third set, the short wavelength-sensitive (SWS) and medium wavelength-sensitive (MWS) cone opsin proteins, was the last to be detected, but materialized in a spatio-temporal pattern reminiscent of the neurogenetic gradient of the cones. These different spatial and temporal patterns indicate that cellular maturation must play a primary role in regulating the onset of expression of some of these proteins, while extrinsic signals must act to coordinate the expression of other proteins across photoreceptors of different ages. |
| Databáze: | OpenAIRE |
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