Characterization and comparative analyses of transcriptomes from the normal and neoplastic human prostate
| Autor: | Nigel Clegg, Denise Abbott, Peter S. Nelson, Camari Ferguson, Roger Coleman |
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| Rok vydání: | 2004 |
| Předmět: |
PCA3
Male Pathology medicine.medical_specialty Transcription Genetic Urology UniGene Biology Polymerase Chain Reaction Transcriptome Prostate Gene expression Databases Genetic medicine Humans Gene Library Expressed Sequence Tags cDNA library Gene Expression Profiling Cancer Prostatic Neoplasms medicine.disease Blotting Northern Gene Expression Regulation Neoplastic medicine.anatomical_structure Real-time polymerase chain reaction Oncology Cancer research Algorithms |
| Zdroj: | The Prostate. 60(3) |
| ISSN: | 0270-4137 |
| Popis: | BACKGROUND The prostate gland is a highly specialized organ with functional attributes that serve to enhance the fertility of mammalian species. Pathological processes affecting the prostate include benign prostate hypertrophy and prostate carcinoma; diseases that account for major morbidity and mortality in middle-aged and elderly men. To facilitate studies of biological processes uniquely represented in the prostate and assess molecular alterations associated with prostate carcinoma, we sought to establish the diversity of gene expression in the normal and neoplastic prostate through the compilation and analysis of a prostate transcriptome. METHODS We assembled and annotated ESTs derived from prostate cDNA libraries that were either produced in our laboratory or available from public sequence repositories such as CGAP, dbEST, and Unigene. Determinations of differential gene expression between the normal prostate, other normal tissues, and neoplastic prostate tissues was performed using statistical algorithms. Confirmation of differential expression was performed by quantitative PCR and Northern analysis. RESULTS A total of 99,448 high-quality ESTs were assembled and annotated to produce a prostate transcriptome comprised of 24,580 distinct TUs. Comparative analyses of gene expression levels identified 61 TUs with exclusive expression in the prostate and 45 TUs with high levels of expression in the prostate relative to at least 25 other normal tissues (P > 0.99). Comparative analyses of ESTs derived from neoplastic prostate tissues identified 75 genes with dysregulated expression in cancer (P > 0.99). CONCLUSIONS The human prostate expresses a diverse repertoire of genes that reflect a functionally complex organ. The identification of genes with prostate-restricted or enhanced expression may provide additional insights into the biochemical processes that interact to form the developmental, signaling, and metabolic pathways of the normal and neoplastic gland. © 2004 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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