Antibiofilm Effects of Macrolide Loaded Microneedle Patches: Prospects in Healing Infected Wounds
Autor: | Saman Zafar, Mohammed Maniruzzaman, Ambreen Akhtar, Zeeshan Ahmad, Muhammad Arshad, Israfil Kucuk, Ming Wei Chang, Aleema Tehreem Zahra, Hussain Zaman, Muhammad Ayaz |
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Rok vydání: | 2020 |
Předmět: |
Male
Staphylococcus aureus Pharmaceutical Science Transdermal Patch 02 engineering and technology Microbial Sensitivity Tests Azithromycin Administration Cutaneous 030226 pharmacology & pharmacy 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Differential scanning calorimetry Drug Delivery Systems Dermis Hyaluronic acid medicine Escherichia coli Animals Humans Pharmacology (medical) Transdermal Skin Pharmacology Wound Healing Organic Chemistry Biofilm Salmonella enterica 021001 nanoscience & nanotechnology Folding endurance Anti-Bacterial Agents Erythromycin Rats Disease Models Animal Drug Liberation medicine.anatomical_structure chemistry Biofilms Wound Infection Molecular Medicine Swelling medicine.symptom 0210 nano-technology Wound healing Biotechnology Biomedical engineering |
Zdroj: | Pharmaceutical research. 38(1) |
ISSN: | 1573-904X |
Popis: | The aim of this study was to fabricate polymeric microneedles, loaded with macrolides (erythromycin, azithromycin), using hyaluronic acid and polyvinyl pyrollidone. These microneedles were fabricated using a vacuum micromolding technique. The integrity of the microneedle patches was studied by recording their morphologic features, folding endurance, swelling and micro-piercing. Physicochemical characteristics were studied by differential scanning calorimetry, thermogravimetric analysis and fourier transform infrared spectroscopy. In-vitro drug release, antibiofilm and effect of microneedle patch on wound healing were also studied to confirm the efficacy of the formulations. Formulated patches displayed acceptable folding endurance (>100) and uniform distribution of microneedles (10 × 10) that can penetrate parafilm. Differential scanning calorimetry results depict a decrease in the crystallinity of macrolides following their incorporation in to a polymer matrix. Percentage release of azithromycin and erythromycin from the polymeric patch formulations (over 30 min) was 90% and 63% respectively. Broadly, the zone of bacterial growth inhibition follows the same order for Staphylococcus aureus, Escherichia coli and Salmonella enterica. After 5 days of treatment with azithromycin patches, the wound healing was complete and skin structure (e.g. hair follicles and dermis) was regenerated. It was concluded that azithromycin loaded microneedle patches can be used to treat biofilms in the infected wounds. |
Databáze: | OpenAIRE |
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