Antibiofilm Effects of Macrolide Loaded Microneedle Patches: Prospects in Healing Infected Wounds

Autor: Saman Zafar, Mohammed Maniruzzaman, Ambreen Akhtar, Zeeshan Ahmad, Muhammad Arshad, Israfil Kucuk, Ming Wei Chang, Aleema Tehreem Zahra, Hussain Zaman, Muhammad Ayaz
Rok vydání: 2020
Předmět:
Male
Staphylococcus aureus
Pharmaceutical Science
Transdermal Patch
02 engineering and technology
Microbial Sensitivity Tests
Azithromycin
Administration
Cutaneous

030226 pharmacology & pharmacy
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Differential scanning calorimetry
Drug Delivery Systems
Dermis
Hyaluronic acid
medicine
Escherichia coli
Animals
Humans
Pharmacology (medical)
Transdermal
Skin
Pharmacology
Wound Healing
Organic Chemistry
Biofilm
Salmonella enterica
021001 nanoscience & nanotechnology
Folding endurance
Anti-Bacterial Agents
Erythromycin
Rats
Disease Models
Animal

Drug Liberation
medicine.anatomical_structure
chemistry
Biofilms
Wound Infection
Molecular Medicine
Swelling
medicine.symptom
0210 nano-technology
Wound healing
Biotechnology
Biomedical engineering
Zdroj: Pharmaceutical research. 38(1)
ISSN: 1573-904X
Popis: The aim of this study was to fabricate polymeric microneedles, loaded with macrolides (erythromycin, azithromycin), using hyaluronic acid and polyvinyl pyrollidone. These microneedles were fabricated using a vacuum micromolding technique. The integrity of the microneedle patches was studied by recording their morphologic features, folding endurance, swelling and micro-piercing. Physicochemical characteristics were studied by differential scanning calorimetry, thermogravimetric analysis and fourier transform infrared spectroscopy. In-vitro drug release, antibiofilm and effect of microneedle patch on wound healing were also studied to confirm the efficacy of the formulations. Formulated patches displayed acceptable folding endurance (>100) and uniform distribution of microneedles (10 × 10) that can penetrate parafilm. Differential scanning calorimetry results depict a decrease in the crystallinity of macrolides following their incorporation in to a polymer matrix. Percentage release of azithromycin and erythromycin from the polymeric patch formulations (over 30 min) was 90% and 63% respectively. Broadly, the zone of bacterial growth inhibition follows the same order for Staphylococcus aureus, Escherichia coli and Salmonella enterica. After 5 days of treatment with azithromycin patches, the wound healing was complete and skin structure (e.g. hair follicles and dermis) was regenerated. It was concluded that azithromycin loaded microneedle patches can be used to treat biofilms in the infected wounds.
Databáze: OpenAIRE