Novel Concept of Micro Patterned Micro Titer Plates Fabricated via UV-NIL for Automated Neuronal Cell Assay Read-Out
| Autor: | Isbaal Ramos, Ana Ayerdi Izquierdo, Clarisa Salado, Nerea Briz Iceta, Mirko Lohse, Manuel W. Thesen, Arne Schleunitz, Anja Haase, Martin Smolka |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Materials science
Fabrication General Chemical Engineering Microfluidics Nanotechnology 02 engineering and technology Article Neuronal cell assay Nanoimprint lithography law.invention lcsh:Chemistry 03 medical and health sciences law R2R UV-NIL General Materials Science Lithography Throughput (business) 030304 developmental biology 0303 health sciences Manufacturing process 021001 nanoscience & nanotechnology Titer Resist lcsh:QD1-999 0210 nano-technology |
| Zdroj: | Nanomaterials TECNALIA Publications Fundación Tecnalia Research & Innovation Nanomaterials, Vol 11, Iss 902, p 902 (2021) Volume 11 Issue 4 |
| Popis: | The UV-nanoimprint lithography(UV-NIL) fabrication of a novel network of micron-sized channels, forming an open channel microfluidic system is described. Details about the complete manufacturing process, from mastering to fabrication in small batches and in high throughput with up to 1200 micro titer plates per hour is presented. Deep insight into the evaluation of a suitable UV-curable material, mr-UVCur26SF is given, presenting cytotoxic evaluation, cell compatibility tests and finally a neuronal assay. The results indicate how the given pattern, in combination with the resist, paves the way to faster, cheaper, and more reliable drug screening. This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement NO. 646260. The research was also partially supported by NextGenMicrofluidics project under HORIZON2020 with grant agreement no 862092. |
| Databáze: | OpenAIRE |
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