Ammonia-oxidizing archaea possess a wide range of cellular ammonia affinities
| Autor: | K. Dimitri Kits, José R. de la Torre, Man-Young Jung, Barbara Bayer, Craig W. Herbold, Graeme W. Nicol, Anna J. Mueller, Linda Hink, Holger Daims, Sung-Keun Rhee, Chloe Wright, Laura E. Lehtovirta-Morley, Christopher J. Sedlacek, Petra Pjevac, Michael Wagner |
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| Přispěvatelé: | Ampère, Département Bioingénierie (BioIng), Ampère (AMPERE), École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-École Centrale de Lyon (ECL), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-École Centrale de Lyon (ECL), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Microbiology
Article Microbial ecology 03 medical and health sciences Ammonia chemistry.chemical_compound Nitrate Ammonium Archaeal physiology Nitrogen cycle Phylogeny Soil Microbiology Ecology Evolution Behavior and Systematics 030304 developmental biology 0303 health sciences Bacteria biology 030306 microbiology Comammox Ammonia monooxygenase biology.organism_classification Archaea Nitrification Metabolism chemistry Environmental chemistry [SDE]Environmental Sciences Oxidation-Reduction |
| Zdroj: | ISME Journal ISME Journal, Nature Publishing Group, 2021, ⟨10.1038/s41396-021-01064-z⟩ The ISME Journal |
| ISSN: | 1751-7362 1751-7370 |
| DOI: | 10.1038/s41396-021-01064-z⟩ |
| Popis: | Nitrification, the oxidation of ammonia to nitrate, is an essential process in the biogeochemical nitrogen cycle. The first step of nitrification, ammonia oxidation, is performed by three, often co-occurring guilds of chemolithoautotrophs: ammonia-oxidizing bacteria (AOB), archaea (AOA), and complete ammonia oxidizers (comammox). Substrate kinetics are considered to be a major niche-differentiating factor between these guilds, but few AOA strains have been kinetically characterized. Here, the ammonia oxidation kinetic properties of 12 AOA representing all major cultivated phylogenetic lineages were determined using microrespirometry. Members of the genus Nitrosocosmicus have the lowest affinity for both ammonia and total ammonium of any characterized AOA, and these values are similar to previously determined ammonia and total ammonium affinities of AOB. This contrasts previous assumptions that all AOA possess much higher substrate affinities than their comammox or AOB counterparts. The substrate affinity of ammonia oxidizers correlated with their cell surface area to volume ratios. In addition, kinetic measurements across a range of pH values supports the hypothesis that—like for AOB—ammonia and not ammonium is the substrate for the ammonia monooxygenase enzyme of AOA and comammox. Together, these data will facilitate predictions and interpretation of ammonia oxidizer community structures and provide a robust basis for establishing testable hypotheses on competition between AOB, AOA, and comammox. |
| Databáze: | OpenAIRE |
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