Primary cilium remodeling mediates a cell signaling switch in differentiating neurons
| Autor: | Raman M. Das, Gabriela Toro-Tapia |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Neural Tube
Cell signaling Cell type animal structures Cellular differentiation Neurogenesis Context (language use) 03 medical and health sciences 0302 clinical medicine medicine Cilia Axon skin and connective tissue diseases Research Articles 030304 developmental biology Neurons 0303 health sciences Multidisciplinary Chemistry Signal Transduction/physiology Cilium Axon extension Neural Tube/metabolism SciAdv r-articles Cell Biology Cell biology Neuroepithelial cell medicine.anatomical_structure nervous system embryonic structures sense organs 030217 neurology & neurosurgery Signal Transduction Research Article Developmental Biology |
| Zdroj: | Toro, G & Das, R 2020, ' Primary cilium remodeling mediates a cell signaling switch in differentiating neurons ', Science Advances . https://doi.org/10.1126/sciadv.abb0601 Toro-Tapia, G & Das, R 2020, ' Primary cilium remodelling mediates a cell signalling switch in differentiating neurons ', Science Advances, vol. 6, no. 21 . https://doi.org/10.1126/sciadv.abb0601 Toro, G & Das, R 2020, ' Primary cilium remodeling mediates a cell signaling switch in differentiating neurons ', Science Advances, vol. 6, no. 21, EABB0601 . https://doi.org/10.1126/sciadv.abb0601 Science Advances |
| Popis: | Newborn neurons assemble a new primary cilium, leading to a change in the mode of Shh signaling that now directs axon extension. Cellular differentiation leads to the formation of specialized cell types and complex morphological variations. Often, differentiating cells transition between states by switching how they respond to the signaling environment. However, the mechanisms regulating these transitions are poorly understood. Differentiating neurons delaminate from the neuroepithelium through the regulated process of apical abscission, which mediates an acute loss of polarity and primary cilium disassembly. Using high-resolution live-cell imaging in chick neural tube, we show that these cells retain an Arl13b+ particle, which elongates and initiates intraflagellar trafficking as it transits toward the cell body, indicating primary cilium remodeling. Notably, disrupting cilia during and after remodeling inhibits axon extension and leads to axon collapse, respectively. Furthermore, cilium remodeling corresponds to a switch from a canonical to noncanonical cellular response to Shh. This work transforms our understanding of how cells can rapidly reinterpret signals to produce qualitatively different responses within the same tissue context. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|