Nasal microbiota clusters associate with inflammatory response, viral load, and symptom severity in experimental rhinovirus challenge
| Autor: | Tommi Kauko, Sofia Männikkö, Liisa Lehtoranta, Markus J. Lehtinen, Sofia D. Forssten, Ronald B. Turner, Buffy Stahl, Ashley A. Hibberd, Sampo J. Lahtinen, Nicolas Yeung, Anna Lyra |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Rhinovirus Science Corynebacterium Nose medicine.disease_cause Article law.invention Feces Young Adult 03 medical and health sciences Probiotic fluids and secretions law otorhinolaryngologic diseases medicine Cluster Analysis Humans Moraxella Inflammation Picornaviridae Infections Multidisciplinary Bacteria biology business.industry Microbiota Biodiversity Viral Load respiratory system biology.organism_classification Bifidobacterium animalis 030104 developmental biology Nasal Swab Immunology Medicine business Viral load Staphylococcus Biomarkers |
| Zdroj: | Scientific Reports Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-018-29793-w |
| Popis: | The role of nasal and fecal microbiota in viral respiratory infections has not been established. We collected nasal swabs and washes, and fecal samples in a clinical study assessing the effect of probiotic Bifidobacterium animalis subsp. lactis Bl-04 on experimental rhinovirus infection. The nasal and fecal microbiota were characterized by 16S rRNA gene sequencing. The resulting data were compared with nasal inflammatory marker concentrations, viral load, and clinical symptoms. By using unsupervised clustering, the nasal microbiota divided into six clusters. The clusters predominant of Staphylococcus, Corynebacterium/Alloiococcus, Moraxella, and Pseudomonadaceae/Mixed had characteristic inflammatory marker and viral load profiles in nasal washes. The nasal microbiota clusters of subjects before the infection associated with the severity of clinical cold symptoms during rhinovirus infection. Rhinovirus infection and probiotic intervention did not significantly alter the composition of nasal or fecal microbiota. Our results suggest that nasal microbiota may influence the virus load, host innate immune response, and clinical symptoms during rhinovirus infection, however, further studies are needed. |
| Databáze: | OpenAIRE |
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