Autor: |
Liyun Wan, Lei, Yong, Liying Yan, Liu, Yue, Pandey, Manish K., Wan, Xia, Varshney, Rajeev K., Jiahai Fang, Boshou Liao |
Rok vydání: |
2020 |
Předmět: |
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DOI: |
10.6084/m9.figshare.12134976 |
Popis: |
Additional file 9. qRT-PCR validation of DEGs between wsc and WT. (A) Transcript levels of 17 genes with average FPKM value ≥2.5. The y-axis showed relative gene expression levels analyzed by qRT-PCR and RNA-Seq. WT qRT-PCR (rose columns) and wsc qRT-PCR (white columns) corresponding to qRT-PCR expression data. WT RNA-Seq (rose lines) and wsc RNA-Seq (gray lines) refer to RNA-seq data. The data presented here are mean values from three repetitions. Error bars represent standard error (SE) (n = 3). (B) Comparison of gene expression ratios from qRT-PCR and RNA-Seq data. RNA-Seq log2 values of the expression ratio (y-axis) are plotted against the three different developmental stages (x-axis). The gene expression was scaled using Z-score of FPKM (mean value of three biological replications) in the heatmap. |
Databáze: |
OpenAIRE |
Externí odkaz: |
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