Aptamer-Conjugated Tb(III)-Doped Silica Nanoparticles for Luminescent Detection of Leukemia Cells
| Autor: | Emil A. Zaripov, Asiya R. Mustafina, Yaroslav A Grechkin, Svetlana L. Grechkina, Maxim V. Berezovski, Svetlana V. Fedorenko |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Aptamer
leukemia cells Medicine (miscellaneous) Nanoparticle 02 engineering and technology Conjugated system silica nanoparticles 010402 general chemistry 01 natural sciences General Biochemistry Genetics and Molecular Biology Article Fluorescence microscope luminescence lcsh:QH301-705.5 Chemistry DNA aptamers 021001 nanoscience & nanotechnology Fluorescence Combinatorial chemistry 0104 chemical sciences lcsh:Biology (General) Magnetic nanoparticles 0210 nano-technology Luminescence probes Biosensor |
| Zdroj: | Biomedicines Volume 8 Issue 1 Biomedicines, Vol 8, Iss 1, p 14 (2020) |
| ISSN: | 2227-9059 |
| Popis: | DNA aptamers have many benefits for cell imaging, such as high affinity and specificity, easiness of chemical functionalization, and low cost of production. Among known aptamers, Sgc8-aptamer was selected against acute lymphoblastic leukemia cells with a dissociation constant in a nanomolar range. The aptamer was previously used for the covalent coupling with fluorescent and magnetic nanoparticles, as well as for the fabrication of aptamer-based biosensors. Among commonly used fluorescent tags, lanthanide nanoparticles offer stable luminescence with narrow, well-resolved emission peaks and the absence of photoblinking. In other words, lanthanide nanoparticles could serve as luminescence reporters and be used in biosensing. In our study, we conjugated amino- and carboxyl-modified silica-coated terbium (III) thiacalix[4]arenesulfonate luminescent nanoparticles with Sgc8-aptamer and showed the ability of the aptamer-conjugated nanoparticles to detect leukemia cells using fluorescence microscopy. In addition, we conducted a cell viability assay and confirmed that the nanoparticles do not induce spontaneous cell apoptosis or necrosis and could be potentially used for bioimaging applications. |
| Databáze: | OpenAIRE |
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