PP123. Evaluation of glycosaminoglycans in placental of pregnant women with and without preeclampsia

Autor: Nelson Sass, R.S. Souza, Thais Alquezar Facca, M.A. Pinhal, Eduado A. Fama
Rok vydání: 2012
Předmět:
Zdroj: Pregnancy Hypertension: An International Journal of Women's Cardiovascular Health. 2:305-306
ISSN: 2210-7789
DOI: 10.1016/j.preghy.2012.04.234
Popis: Introduction Preeclampsia (PE) is a major cause of maternal and perinatal mobility and mortality and its etiology is not yet completely understood. Recently studies have shown the association between increased expression of glycosaminoglycans (GAGs) in placental of women with PE and its physiopathology. Objectives Identify and quantify GAGs in placental of pregnant women in healthy pregnancy and PE. Methods Case-control study with 44 patients, control group (CG) n = 29 and n = 15 PE group. All patients were submitted to placental tissue resection (sample size of 5 × 5 cm with the umbilical cord insertion in the center). The tissue was conserved in acetone. The GAGs’ analysis consisted of centrifugation, proteolysis, precipitation, and electrophoresis Results Average age and gestational age in CG and PE were 27.33 years and 39.02 weeks and 24.17 and 36.90, respectively. In CG 68.96% (20/29) were Caucasian and 80.00% (12/15) in PE. We found in CG 34.48% (10/29) of primiparous and 40.00% (6/15) in PE. The average 24-hour proteinuria in PE was 554.28 g/24hs. The average birth weight was 3333.31 g in CG and 2972.66 in PE. The mean ± standard deviation of dermatan sulfate (DS), heparan sulfate (HS) and hyaluronic acid (HA) in CG and PE were: 0,100 μg/mg of tissue ±0,005 and 0,144 ±0,071; 0,077 ± 0,041 and 0,113 ± 0,061; 1,281 ± 1,857 and 3,076 ± 4,930. Conclusion GAGs are increased in PE when compared to normal pregnancy. The expression of HA and HS was twice higher in PE. More studies are needed to determine the correlation between GAGs and physiopathology of PE.
Databáze: OpenAIRE
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