Identification of new actin-associated polypeptides that are modified by viral transformation and changes in cell shape
| Autor: | Claire Shapland, Durward Lawson, Paul Lowings |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1988 |
| Předmět: |
Stress fiber
Fluorescent Antibody Technique Simian virus 40 Cycloheximide Biology Microfilament Epitope Chromatography Affinity Cell Line chemistry.chemical_compound Adherent Culture Animals Humans Cytoskeleton Actin Cells Cultured Immunoassay Cell Biology Articles Fibroblasts Cell Transformation Viral Molecular biology Actins Rats Actin Cytoskeleton chemistry Avian Sarcoma Viruses Cell culture Electrophoresis Polyacrylamide Gel Peptides |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | By using a monoclonal antibody we have identified a new polypeptide doublet (C4h and C4l) of Mr approximately 21 kD and pI 8 and 7, respectively, that is associated with and (at the immunofluorescence level) uniformly distributed on actin filament bundles in rat, mouse, and other vertebrate species. C4 is absent in neurones, erythrocytes, and skeletal muscle but the epitope is evolutionarily conserved as it is present in invertebrates such as molluscs and crustaceans. C4h is not found in cells such as lymphocytes and oncogenically transformed mesenchymal cells where actin stress fiber bundles are reduced in number or absent. C4l, on the other hand, is always present. C4h expression can also be blocked by switching normal nontransformed mesenchymal cells from adherent to suspension culture. Reexpression of C4h occurs 24 h after these cells are returned to normal adherent culture conditions, but can be blocked by either actinomycin D or cycloheximide, suggesting that the expression of this epitope is regulated at the transcriptional level. |
| Databáze: | OpenAIRE |
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