Solid lipid nanoparticles for ocular delivery of isoniazid: evaluation, proof of concept and in vivo safetykinetics
| Autor: | Cheerneni S. Srinivas, Mandeep Singh, Indu Pal Kaur, Afzal Hussain, Ana Guzman-Aranguez |
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| Rok vydání: | 2019 |
| Předmět: |
Bioquímica
Magnetic Resonance Spectroscopy genetic structures Swine Biomedical Engineering Medicine (miscellaneous) Bioengineering 02 engineering and technology Development Pharmacology Eye Microscopy Atomic Force Cornea 03 medical and health sciences Drug Delivery Systems Pharmacokinetics Microscopy Electron Transmission X-Ray Diffraction In vivo Solid lipid nanoparticle Spectroscopy Fourier Transform Infrared medicine Isoniazid Animals General Materials Science 030304 developmental biology 0303 health sciences Drug Carriers Chemistry Area under the curve Anatomía ocular bacterial infections and mycoses 021001 nanoscience & nanotechnology eye diseases In vitro Rats body regions Toxicity sense organs Rabbits 0210 nano-technology Ex vivo medicine.drug |
| Zdroj: | E-Prints Complutense. Archivo Institucional de la UCM instname E-Prints Complutense: Archivo Institucional de la UCM Universidad Complutense de Madrid |
| ISSN: | 1748-6963 |
| Popis: | Aim: Evaluation of solid lipid nanoparticles (SLNs) for ocular delivery of isoniazid (INH). Materials & methods: INH-SLNs were characterized for morphological, thermal, crystalline and nuclear magnetic resonance properties. In vitro release and ex vivo corneal permeability of INH-SLNs was also evaluated. Proof-of-concept uptake studies were performed in corneal and conjunctival cell lines and in vivo in rat eye using fluorescein-labeled SLNs. Antimycobacterial activity of INH-SLNs was confirmed. In vivo aqueous humor pharmacokinetics, toxicity and tolerance was performed in rabbit/rat eye. Results: INH-SLNs showed extended release (48 h), enhanced corneal permeability (1.6-times), five-times lower MIC, significant in vitro and in vivo uptake of fluorescein-labeled SLNs, 4.2-times ocular bioavailability (area under the curve) and in vivo acute and repeat dose safety. Conclusion: INH-SLNs are an effective ocular delivery system. |
| Databáze: | OpenAIRE |
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