Partial correction of abnormal cardiac development in caspase-8-deficient mice by cardiomyocyte expression of p 35
| Autor: | Shu-ichi Yamada, Kazuhiro Sakamaki, Shin Yonehara, Shinya Toyokuni, Takayuki Morisaki, Nobuyuki Yajima |
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| Rok vydání: | 2004 |
| Předmět: |
Genetically modified mouse
Heart Defects Congenital Male Programmed cell death Transgene Green Fluorescent Proteins DNA Recombinant Drug Resistance Gene Expression Apoptosis Mice Transgenic Caspase 8 Mice Viral Proteins Pregnancy Genetics medicine Staurosporine Animals Humans Myocytes Cardiac Heart formation Caspase Cells Cultured Mice Knockout Mice Inbred C3H biology Base Sequence Molecular biology Caspase Inhibitors Recombinant Proteins Cell biology Mice Inbred C57BL Caspases biology.protein Animal Science and Zoology Female Agronomy and Crop Science Biotechnology medicine.drug HeLa Cells |
| Zdroj: | Transgenic research. 14(5) |
| ISSN: | 0962-8819 |
| Popis: | Baculovirus p 35 protein protects cells from apoptotic cell death by inhibiting caspase activation. We have established transgenic mouse lines specifically expressing p 35 in cardiomyocytes, and primary cardiomyocytes isolated from these mice exhibit resistance to staurosporine-induced apoptosis. In a previous study, we observed defects in heart formation associated with abdominal hemorrhage and cardiomyocyte cell death in caspase-8-deficient animals. In order to better understand the etiology of the cardiac defects and embryonic lethality in caspase-8-deficient mice, we crossed these mice with the p 35 transgenic animals. Although the newly generated mice still died in utero and exhibited some cardiac defects, cardiomyocyte apoptosis was suppressed and ventricular trabeculation was restored. Thus, cardiomyocyte expression of p 35 prevented cell death induced by staurosporine or caspase-8 deficiency. Additionally, our data suggest that caspase-8 plays multiple roles in cardiac development. |
| Databáze: | OpenAIRE |
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