Klebsiella pneumoniae ST307 with OXA-181: threat of a high-risk clone and promiscuous plasmid in a resource-constrained healthcare setting
| Autor: | A C Stoltz, Johann D.D. Pitout, Gisele Peirano, Nontombi Marylucy Mbelle, Liang Chen, Marthie M. Ehlers, Kathy-Anne Strydom, Michelle Lowe, Barry N. Kreiswirth, Marleen M. Kock, Diego B. Nobrega |
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| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
clone (Java method) Lineage (genetic) Klebsiella pneumoniae Biology beta-Lactamases law.invention South Africa Plasmid Bacterial Proteins law Humans Pharmacology (medical) Clade Polymerase chain reaction Original Research Pharmacology Whole genome sequencing Outbreak biology.organism_classification Virology Clone Cells Klebsiella Infections Infectious Diseases Delivery of Health Care Plasmids |
| Zdroj: | J Antimicrob Chemother |
| ISSN: | 1460-2091 0305-7453 |
| DOI: | 10.1093/jac/dkz550 |
| Popis: | Introduction Klebsiella pneumoniae with OXA-48-like enzymes were introduced into Tshwane Tertiary Hospital (TTH) (Pretoria, South Africa) during September 2015, causing nosocomial outbreaks. Methods PCR methodologies and WGS were used to characterize K. pneumoniae with carbapenemases (n = 124) from TTH (July 2015–December 2016). Results PCR was used to track K. pneumoniae ST307 with OXA-181 among 60% of carbapenemase-positive isolates in different wards/units over time and showed the transmission of IncX3 plasmids to other K. pneumoniae clones. WGS identified different ST307 clades: 307_OXA181 (consisting of two lineages, A and B) with OXA-181 on IncX3 plasmids (named p72_X3_OXA181) and clade 307_VIM with VIM-1 on IncFII plasmids. Clade 307_OXA181 lineage B was responsible for the rapid increase and transmission of OXA-181 K. pneumoniae in various wards/units throughout TTH, while the numbers of clade 307_OXA181 lineage A and clade 307_VIM remained low. Separate outbreaks were due to K. pneumoniae ST17 and ST29 with p72_X3_OXA181 plasmids. Conclusions The high-risk clone K. pneumoniae ST307 with OXA-181 rapidly spread to different wards/units despite infection and prevention measures. ST307 clades and lineages seemingly acted differently in outbreak situations. This study also highlighted the threat of promiscuous plasmids such as p72_X3_OXA181. |
| Databáze: | OpenAIRE |
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