Osteoclast formation, inflammation, and matrix metalloproteinase-9 are downregulated in bone repair following root canal treatment in dogs teeth
| Autor: | Marina Fernandes de Sena, Paulo Nelson-Filho, Renato Petille, Giuliana Campos Chaves Lamarque, Francisco Wanderley Garcia Paula-Silva, Léa Assed Bezerra da Silva, Maya Fernanda Manfrin Arnez, Fernanda Regina Ribeiro-Santos |
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| Rok vydání: | 2020 |
| Předmět: |
Pathology
medicine.medical_specialty Root canal Osteoclasts Inflammation Bone healing Calcium Hydroxide Root Canal Filling Materials 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Dogs Osteoclast medicine Animals General Dentistry Periodontitis Calcium hydroxide Root Canal Irrigants business.industry 030206 dentistry medicine.disease Root Canal Therapy medicine.anatomical_structure chemistry Matrix Metalloproteinase 9 030220 oncology & carcinogenesis Immunohistochemistry Alkaline phosphatase medicine.symptom Dental Pulp Cavity business Periapical Periodontitis |
| Zdroj: | Clinical oral investigations. 25(7) |
| ISSN: | 1436-3771 |
| Popis: | The aim of this study was to investigate the inflammatory infiltrate, osteoclast formation, and expression of MMP-9 during the healing phase following root canal treatment in teeth with apical periodontitis. Apical periodontitis was induced in dogs teeth, and root canal treatment was performed in a single visit or using calcium hydroxide as intracanal medication. One hundred and eighty days following treatment the presence of inflammation was examined, and the tissues were stained to detect osteoclasts by means of a tartrate resistant alkaline phosphatase (TRAP) assay. Synthesis of MMP-9 was detected using Western blotting and immunohistochemistry. Teeth with apical periodontitis that had root canal therapy performed in a single visit presented a higher synthesis of MMP-9 compared with root canal treatment using calcium hydroxide. Treatment with calcium hydroxide resulted in a reduced amount of inflammatory cells and MMP-9 positive cells. Osteoclast formation, the number of MMP-9 positive osteoclasts and cementocytes, was reduced following root canal treatment, regardless of the root canal treatment protocol used. Root canal treatment reduced the amount of inflammatory cells and osteoclasts in periapical area. The use of calcium hydroxide as intracanal medication resulted in a lower synthesis of MMP-9, though the number of osteoclasts and MMP-9 positive osteoclasts were similar between the groups. Periapical bone repair following root canal treatment is impacted by therapy performed either in single visit or using calcium hydroxide dressing measured by inflammatory cell recruitment, osteoclast formation, and MMP-9 synthesis. |
| Databáze: | OpenAIRE |
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