Alternative lipid remodelling pathways for glycosylphosphatidylinositol membrane anchors in Saccharomyces cerevisiae

Autor: Christine Vionnet, György Sipos, Fulvio Reggiori, Andreas Conzelmann
Rok vydání: 1997
Předmět:
Phosphatidylinositols/metabolism
Ceramide
Saccharomyces cerevisiae Proteins
Glycosylphosphatidylinositols
Saccharomyces cerevisiae
Biology
Phosphatidylinositols
General Biochemistry
Genetics and Molecular Biology
Saccharomyces cerevisiae/genetics
Fungal Proteins
symbols.namesake
chemistry.chemical_compound
Phosphatidylinositol
Protein Precursors
Molecular Biology
Diacylglycerol kinase
chemistry.chemical_classification
General Immunology and Microbiology
General Neuroscience
Endoplasmic reticulum
Fatty Acids
Dyneins
Fatty acid
Lipid signaling
Golgi apparatus
Lipid Metabolism
Protein Precursors/metabolism
Glycosylphosphatidylinositols/metabolism
Cell biology
Fungal Proteins/genetics
chemistry
Membrane protein
Biochemistry
Mutation
symbols
lipids (amino acids
peptides
and proteins)
Acyltransferases
Research Article
Subcellular Fractions
Zdroj: Sipos, G, Reggiori, F, Vionnet, C & Conzelmann, A 1997, ' Alternative lipid remodelling pathways for glycosylphosphatidylinositol membrane anchors in Saccharomyces cerevisiae ', The EMBO Journal, vol. 16, no. 12, pp. 3494-505 . https://doi.org/10.1093/emboj/16.12.3494
ISSN: 1460-2075
DOI: 10.1093/emboj/16.12.3494
Popis: Glycosylphosphatidylinositol (GPI)-anchored membrane proteins of Saccharomyces cerevisiae exist with two types of lipid moiety--diacylglycerol or ceramide--both of which contain 26:0 fatty acids. To understand at which stage of biosynthesis these long-chain fatty acids become incorporated into diacylglycerol anchors, we compared the phosphatidylinositol moieties isolated from myo-[2-(3)H]inositol-labelled protein anchors and from GPI intermediates. There is no evidence for the presence of long-chain fatty acids in any intermediate of GPI biosynthesis. However, GPI-anchored proteins contain either the phosphatidylinositol moiety characteristic of the precursor lipids or a version with a long-chain fatty acid in the sn-2 position of glycerol. The introduction of long-chain fatty acids into sn-2 occurs in the endoplasmic reticulum (ER) and is independent of the sn-2-specific acyltransferase SLC1. Analysis of ceramide anchors revealed the presence of two types of ceramide, one added in the ER and another more polar molecule which is found only on proteins which have reached the mid Golgi. In summary, the lipid of GPI-anchored proteins can be exchanged by at least three different remodelling pathways: (i) remodelling from diacylglycerol to ceramide in the ER as proposed previously; (ii) remodelling from diacylglycerol to a more hydrophobic diacylglycerol with a long-chain fatty acid in sn-2 in the ER; and (iii) remodelling to a more polar ceramide in the Golgi.
Databáze: OpenAIRE
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