Diurnal Expression of the Per2 Gene and Protein in the Lateral Habenular Nucleus
| Autor: | Haiyan Xu, Jinyu Xiao, Li Mu, Yongmao Liu, Hua Zhao, Zhigong Zhao |
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| Rok vydání: | 2015 |
| Předmět: |
Male
circadian rhythm endocrine system medicine.medical_specialty Photoperiod Biology Article Catalysis lcsh:Chemistry Inorganic Chemistry running-wheel activity Internal medicine Gene expression Zeitgeber medicine Animals RNA Messenger Circadian rhythm Rats Wistar Physical and Theoretical Chemistry lcsh:QH301-705.5 Molecular Biology Spectroscopy Regulation of gene expression Habenula Suprachiasmatic nucleus suprachiasmatic nucleus Organic Chemistry Period Circadian Proteins General Medicine period2 gene Computer Science Applications PER2 Endocrinology lcsh:Biology (General) lcsh:QD1-999 Gene Expression Regulation lateral habenular nucleus hormones hormone substitutes and hormone antagonists |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 16, Iss 8, Pp 16740-16749 (2015) Volume 16 Issue 8 Pages 16740-16749 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms160816740 |
| Popis: | The suprachiasmatic nucleus plays an important role in generating circadian rhythms in mammals. The lateral habenular nucleus (LHb) is closely linked to this structure. Interestingly, the LHb shows a rhythmic firing rate in vivo and in vitro, and sustained oscillation of rhythmic genes in vitro. However, under the in vivo condition, whether rhythmic gene expression in the LHb has circadian rhythms remains unknown. In this study, we examined LHb tissue in rats to determine Period2 (Per2) gene and protein expression at six zeitgeber time points (ZT2, ZT6, ZT10, ZT14, ZT18, and ZT22) in a 12-h light and 12-h dark (LD) environment. We found that in the LD environment, Per2 gene expression and PER2 protein levels in the LHb were higher in the day and lower in the night, showing periodic oscillation, with a peak at ZT10 and a trough at ZT22 (Per2 mRNA) and ZT18 (PER2 protein). We conclude that Per2 expression and PER2 protein levels in the LHb have rhythmic oscillation in vivo. This study provides a basis for further study on the role of the LHb in the circadian rhythm system. |
| Databáze: | OpenAIRE |
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