High extracellular induced sputum haem oxygenase-1 in sarcoidosis and chronic beryllium disease
| Autor: | Nurdan Kokturk, Elizabeth Fireman, J. Grief, M. Stark, M. Sabag |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Adult
Male medicine.medical_specialty Systemic disease Sarcoidosis Clinical Biochemistry medicine.disease_cause Biochemistry Gastroenterology digestive system Pulmonary function testing chemistry.chemical_compound Berylliosis Reference Values Internal medicine Extracellular Medicine Humans Nitrite Retrospective Studies biology business.industry Sputum General Medicine Middle Aged medicine.disease digestive system diseases Up-Regulation Ferritin Oxidative Stress surgical procedures operative chemistry Immunology biology.protein Female business Oxidative stress CD8 Heme Oxygenase-1 |
| Zdroj: | European journal of clinical investigation. 39(7) |
| ISSN: | 1365-2362 |
| Popis: | Background Sarcoidosis and chronic beryllium disease (CBD) are inflammatory conditions in which oxidative stress state may be crucial for disease outcome. This study compares haem oxygenase-1 (HO-1) extracellular activity for the first time in patients with sarcoidosis or CBD and in healthy controls. Materials and methods Induced sputum was recovered using a standard protocol. Pulmonary function tests (PFT) were performed by conventional methods. T lymphocyte subsets (CD4 and CD8) were measured by flow activated cell shorter (FACS). The HO-1 and nitrite levels were measured by a bilirubin-biliverdin reductase-dependant reaction and Greiss reaction respectively. Ferritin and iron levels were measured by enzymatic reaction and chemiluminometric immunoassay respectively. Results The mean percentage of lymphocytes was significantly higher in the 36 sarcoid patients compared with that in the 17 controls (P = 0·001). The mean CD4/CD8 ratio was significantly higher in the sarcoid and the 10 CBD patients compared to that in controls (P = 0·000 and 0·002 respectively), as was the mean HO-1 activity (P = 0·045 and 0·041 respectively). The HO-1 activity did not differ with the sarcoidosis stage. The HO-1 level and PFT parameters were negatively correlated. The differences in mean nitrite, ferritin and iron levels were non-significant between the three groups. The HO-1 and ferritin levels were correlated (P = 0·008). Conclusions We succeeded in non-invasively measuring the activity of HO-1 from cells of airways in spite of its being an intracellular enzyme. The HO-1 levels in sarcoidosis and CBD were abnormally elevated. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|