The E3 ubiquitin ligase MIB-1 is necessary to form the nuclear halo in C. elegans sperm
| Autor: | Leslie A Herrera, Daniel A. Starr |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0303 health sciences
030302 biochemistry & molecular biology Nonsense mutation Notch signaling pathway Halo nucleus Biology Sperm Germline Forward genetics digestive system diseases Green fluorescent protein Cell biology Ubiquitin ligase 03 medical and health sciences biology.protein neoplasms 030304 developmental biology |
| DOI: | 10.1101/301291 |
| Popis: | Unlike the classical nuclear envelope with two membranes found in other eukaryotic cells, most nematode sperm nuclei are not encapsulated by membranes. Instead, they are surrounded by a nuclear halo of unknown composition. How the halo is formed and regulated is unknown. We used forward genetics to identify molecular lesions behind three classicalfer(fertilization defective) mutations that disrupt the ultrastructure of theC. eleganssperm nuclear halo. We foundfer-2andfer-4alleles to be nonsense mutations inmib-1.fer-3was caused by a nonsense mutation ineri-3. GFP::MIB-1 was expressed in the germline during early spermatogenesis, but not in mature sperm.mib-1encodes a conserved E3 ubiquitin ligase homologous to vertebrate Mib1 and Mib2, which function in Notch signaling. However,C. elegans mib-1has no Notch-related phenotypes. Thus,mib-1has been co-opted to regulate the formation of the nuclear halo during nematode spermatogenesis. |
| Databáze: | OpenAIRE |
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