Immune stimuli shape the small non-coding transcriptome of extracellular vesicles released by dendritic cells

Autor: Driedonks, Tom A P, van der Grein, Susanne G, Ariyurek, Yavuz, Buermans, Henk P J, Jekel, Henrike, Chow, Franklin W N, Wauben, Marca H M, Buck, Amy H, 't Hoen, Peter A C, Nolte-'t Hoen, Esther N M, LS Celbiologie-Algemeen, dB&C I&I
Rok vydání: 2018
Předmět:
Lipopolysaccharides
0301 basic medicine
Small RNA
Bone Marrow Cells
Extracellular RNA
Transcriptome
Extracellular Vesicles
Mice
03 medical and health sciences
Cellular and Molecular Neuroscience
All institutes and research themes of the Radboud University Medical Center
RNA
Transfer
Immune suppression
microRNA
Animals
RNA
Small Nucleolar
Small nucleolar RNA
Molecular Biology
Cells
Cultured
Cholecalciferol
Fluorescent Dyes
Ribonucleoprotein
Pharmacology
Immune activation
Sequence Analysis
RNA
Chemistry
RNA
Dendritic Cells
Biomarker
Cell Biology
Cell biology
Mice
Inbred C57BL
MicroRNAs
Microscopy
Electron
030104 developmental biology
Nanoparticles
RNA
Small Untranslated
Molecular Medicine
Original Article
Small RNA sequencing
Nanomedicine Radboud Institute for Molecular Life Sciences [Radboudumc 19]
Small nuclear RNA
Zdroj: Cellular and Molecular Life Sciences, 75, 20, pp. 3857-3875
Cellular and Molecular Life Sciences, 75(20), 2857. Birkhauser Verlag Basel
Cellular and Molecular Life Sciences
Cellular and Molecular Life Sciences, 75, 3857-3875
ISSN: 1420-9071
1420-682X
DOI: 10.1007/s00018-018-2842-8
Popis: The release and uptake of nano-sized extracellular vesicles (EV) is a highly conserved means of intercellular communication. The molecular composition of EV, and thereby their signaling function to target cells, is regulated by cellular activation and differentiation stimuli. EV are regarded as snapshots of cells and are, therefore, in the limelight as biomarkers for disease. Although research on EV-associated RNA has predominantly focused on microRNAs, the transcriptome of EV consists of multiple classes of small non-coding RNAs with potential gene-regulatory functions. It is not known whether environmental cues imposed on cells induce specific changes in a broad range of EV-associated RNA classes. Here, we investigated whether immune-activating or -suppressing stimuli imposed on primary dendritic cells affected the release of various small non-coding RNAs via EV. The small RNA transcriptomes of highly pure EV populations free from ribonucleoprotein particles were analyzed by RNA sequencing and RT-qPCR. Immune stimulus-specific changes were found in the miRNA, snoRNA, and Y-RNA content of EV from dendritic cells, whereas tRNA and snRNA levels were much less affected. Only part of the changes in EV-RNA content reflected changes in cellular RNA, which urges caution in interpreting EV as snapshots of cells. By comprehensive analysis of RNA obtained from highly purified EV, we demonstrate that multiple RNA classes contribute to genetic messages conveyed via EV. The identification of multiple RNA classes that display cell stimulation-dependent association with EV is the prelude to unraveling the function and biomarker potential of these EV-RNAs. Electronic supplementary material The online version of this article (10.1007/s00018-018-2842-8) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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