miR-18a Inhibitor Suppresses Leukemia Cell Proliferation by Upregulation of PTEN Expression
| Autor: | Liping Zhang, Lingxia Kong, Yuzhi Yang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
THP-1 Cells
Cell Apoptosis 030204 cardiovascular system & hematology Transfection 03 medical and health sciences 0302 clinical medicine Lab/In Vitro Research medicine PTEN Humans THP1 cell line Neoplasm Metastasis Protein kinase B PI3K/AKT/mTOR pathway Cell Proliferation Leukemia biology Chemistry Cell growth Gene Expression Regulation Leukemic Microscopy Ultraviolet PTEN Phosphohydrolase General Medicine medicine.disease Up-Regulation MicroRNAs medicine.anatomical_structure 030220 oncology & carcinogenesis biology.protein Cancer research |
| Zdroj: | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research |
| ISSN: | 1643-3750 |
| Popis: | BACKGROUND Leukemia is common in aging adults and has very high mortality worldwide. The present study was designed to investigate the therapeutic efficacy of miR-18a inhibitor against WEHI-3 and THP-1 leukemia cells. MATERIAL AND METHODS The changes in miR-18a inhibitor-transfected WEHI-3 and THP-1 cell proliferative potential was measured by use of the Cell Counting Kit-8 assay. Apoptotic changes were analyzed by electron microscopy, and evaluation of PI3K, AKT, mTOR, and PTEN expression was assessed by RT-qPCR assay. RESULTS Transfection of miR-18a inhibitor significantly (P |
| Databáze: | OpenAIRE |
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