Analysis of Hippo and TGFβ signaling in polarizing epithelial cells and mouse embryos
| Autor: | Liliana Attisano, Masahiro Narimatsu, Jeffrey L. Wrana, B. Labibi |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
endocrine system Cancer Research animal structures Morphogenesis Embryonic Development Cell Cycle Proteins Smad Proteins SMAD Protein Serine-Threonine Kinases Mice 03 medical and health sciences 0302 clinical medicine Transforming Growth Factor beta Cell polarity Animals Hippo Signaling Pathway Molecular Biology Transcription factor Adaptor Proteins Signal Transducing Hippo signaling pathway biology fungi Cell Polarity Epithelial Cells YAP-Signaling Proteins Cell Biology Transforming growth factor beta Phosphoproteins Cell biology body regions Crosstalk (biology) 030104 developmental biology biology.protein sense organs Signal transduction Acyltransferases 030217 neurology & neurosurgery Signal Transduction Transcription Factors Developmental Biology |
| Zdroj: | Differentiation. 91:109-118 |
| ISSN: | 0301-4681 |
| DOI: | 10.1016/j.diff.2016.01.003 |
| Popis: | The Hippo signaling pathway is involved in numerous biological events ranging from early development to organogenesis and when disrupted, impacts various human diseases including cancer. The Hippo pathway also interacts with and controls the activity of other signaling pathways such as the TGFβ/Smad pathway, in which Hippo pathway activity influences the subcellular localization of Smad transcription factors. Here, we describe techniques for examining crosstalk between Hippo and TGFβ signaling in polarizing mammary epithelial cells. In addition, we provide detailed methods for analyzing the subcellular localization of the Hippo pathway effectors, Taz and Yap using both in vitro cultured epithelial cells and in vivo in pregastrulation mouse embryos. |
| Databáze: | OpenAIRE |
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