Effects of miR-126 on the STAT3 signaling pathway and the regulation of malignant behavior in lung cancer cells
| Autor: | Jian Cheng, Ji-hua Wang, Zaiyun Zhang, Xiaoming Yu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cancer Research Cell Flow cytometry malignant biological behavior 03 medical and health sciences 0302 clinical medicine STAT3 signal pathway medicine STAT3 non-small cell lung cancer A549 cell Oncogene biology medicine.diagnostic_test Chemistry Articles Transfection miR-126 Cell cycle Molecular biology 030104 developmental biology medicine.anatomical_structure Oncology Apoptosis 030220 oncology & carcinogenesis biology.protein |
| Zdroj: | Oncology Letters |
| ISSN: | 1792-1082 1792-1074 |
| DOI: | 10.3892/ol.2018.8360 |
| Popis: | The functions of miR-126-mediated signal transducers and activators of the transcription 3 (STAT3) signal pathway were investigated in regulating the behavior of cells in non-small cell lung cancer (NSCLC). Cultured NSCLC A549 cells were transfected with empty, miR-126 overexpression or miR-126 knocked-down expression plasmids. After transfection efficiency verification by reverse transcription polymerase chain reaction (RT-PCR) and culture for 24 h, methyl thiazolyl tetrazolium (MTT) was applied to detect cell proliferation rate, migration distance was measured in scratch assays, cell cycle was determined through flow cytometry, the mRNA expression level of caspase-3 in cells was detected using RT-PCR and protein expression levels of STAT3 were detected using western blotting. Our results showed the cell proliferation rate was significantly higher in cells of the overexpression group than that in those of the control group (p |
| Databáze: | OpenAIRE |
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