Isolation and analysis of extracellular vesicles in a Morpho butterfly wing-integrated microvortex biochip
| Autor: | Yueshuang Xu, Jie Sun, Yufeng Liu, Renjie Chai, Weiguo Tao, Yuanjin Zhao, Shanying Han |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Lipid Bilayers
Microfluidics Biomedical Engineering Biophysics Nanoprobe Biosensing Techniques 02 engineering and technology 01 natural sciences Extracellular Vesicles Lab-On-A-Chip Devices Electrochemistry Animals Wings Animal Biochip Lipid bilayer biology Chemistry Morpho menelaus 010401 analytical chemistry Membrane structure Morpho General Medicine Extracellular vesicle 021001 nanoscience & nanotechnology biology.organism_classification Lipids Nanostructures 0104 chemical sciences 0210 nano-technology Butterflies Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 154:112073 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2020.112073 |
| Popis: | With the function of mediating intercellular communication between cells, extracellular vesicles (EVs) have been intently studied for their physiopathology and clinical application values. However, efficient EV isolation from biological fluids remains a significant challenge. To address this, this work constructs a new microvortex chip that can isolate EVs efficiently by integrating the lipid nanoprobe modified Morpho Menelaus (M. Menelaus) butterfly wing into microfluidic chip. M. Menelaus wing is well known for its orderly arranged periodic nanostructures and can generate microvortex when liquid passes through it, leading to increased interaction between EVs and M. Menelaus wing. In addition, the nanoprobe containing lipid tails can be inserted into EVs through their lipid bilayer membrane structure. Based on the described properties, high-throughput enrichment of EVs with over 70% isolation efficiency was realized. Moreover, it was demonstrated that the nanoprobe system based on M. Menelaus wing enabled downstream biological analysis of nucleic acids and proteins in EVs. Microvortex chips showed potential application value in efficient EV isolation for biomedical research and cancer diagnosis. |
| Databáze: | OpenAIRE |
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