Fast, Ultrasensitive Detection of Reactive Oxygen Species Using a Carbon Nanotube Based-Electrocatalytic Intracellular Sensor
| Autor: | Nga-Chi Yip, Paula M. Mendes, David J. Garrett, Simon K. Jackson, Alison J. Downard, Kim Baronian, Jacqueline M. Hicks, György Fejer, Frankie J. Rawson, Wondwossen Abate, Nicholas J.F. Dodd |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Lipopolysaccharides
Materials science macrophage cells hydrogen peroxide 02 engineering and technology Biosensing Techniques Electrocatalyst 03 medical and health sciences chemistry.chemical_compound Mice electrocatalytic sensor Animals General Materials Science Hydrogen peroxide intracellular sensor 030304 developmental biology chemistry.chemical_classification reactive oxygen species 0303 health sciences Toll-like receptor Reactive oxygen species NADPH oxidase Innate immune system biology carbon nanotubes Nanotubes Carbon Macrophages NADPH Oxidases 021001 nanoscience & nanotechnology Toll-Like Receptor 4 chemistry Biochemistry Biophysics biology.protein Signal transduction 0210 nano-technology Intracellular Intracellular Sensor Electrocatalytic Sensor Carbon Nanotubes Reactive Oxygen Species Hydrogen Peroxide Macrophage Cells Research Article |
| Zdroj: | ACS Applied Materials & Interfaces |
| ISSN: | 1944-8252 1944-8244 |
| Popis: | Herein, we report a highly sensitive electrocatalytic sensor-cell construct that can electrochemically communicate with the internal environment of immune cells (e.g., macrophages) via the selective monitoring of a particular reactive oxygen species (ROS), hydrogen peroxide. The sensor, which is based on vertically aligned single-walled carbon nanotubes functionalized with an osmium electrocatalyst, enabled the unprecedented detection of a local intracellular “pulse” of ROS on a short second time scale in response to bacterial endotoxin (lipopolysaccharide-LPS) stimulation. Our studies have shown that this initial pulse of ROS is dependent on NADPH oxidase (NOX) and toll like receptor 4 (TLR4). The results suggest that bacteria can induce a rapid intracellular pulse of ROS in macrophages that initiates the classical innate immune response of these cells to infection. |
| Databáze: | OpenAIRE |
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