Multiplexed programmable release of captured DNA
| Autor: | Lloyd M. Smith, Julia Kennedy-Darling, Matthew T. Holden, Michael R. Shortreed |
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| Rok vydání: | 2014 |
| Předmět: |
Oligonucleotides
Computational biology Biology Real-Time Polymerase Chain Reaction Biochemistry Article Nucleic acid thermodynamics chemistry.chemical_compound Sequencing by hybridization Molecular Biology Fluorescent Dyes Oligonucleotide Array Sequence Analysis Genetics Oligonucleotide Organic Chemistry RNA Ribosomal 5S Nucleic Acid Hybridization DNA Branch migration Chromatin chemistry Complementary sequences RNA Ribosomal Molecular Medicine DNA microarray |
| Zdroj: | Chembiochem : a European journal of chemical biology. 15(16) |
| ISSN: | 1439-7633 |
| Popis: | Nucleic-acid hybridization is widely used for the specific capture of complementary sequences from complex samples. It is useful for both analytical methodologies, such as array hybridization (e.g. transcriptome analysis, genetic-variation analysis), and preparative strategies such as exome sequencing and sequence-specific proteome capture and analysis (PICh, HyCCAPP). It has not generally been possible to selectively elute particular captured subsequences, however, as the conditions employed for disruption of a duplex can lack the specificity needed to discriminate between different sequences. We show here that it is possible to bind and selectively release multiple sets of sequences by using toehold-mediated DNA branch migration. The strategy is illustrated for simple mixtures of oligonucleotides, for the sequence-specific capture and specific release of crosslinked yeast chromatin, and for the specific release of oligonucleotides hybridized to DNA microarrays. |
| Databáze: | OpenAIRE |
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