Vasopressin mRNA Expression in Individual Magnocellular Neuroendocrine Cells of the Supraoptic and Paraventricular Nucleus in Response to Water Deprivation
| Autor: | James N. Hayward, Rick B. Meeker, Robert S. Greenwood |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Male
medicine.medical_specialty Vasopressin Vasopressins Endocrinology Diabetes and Metabolism Molecular Sequence Data Population Neuropeptide In situ hybridization Biology Supraoptic nucleus Cellular and Molecular Neuroscience Endocrinology Internal medicine medicine Animals RNA Messenger education Neurons education.field_of_study Base Sequence Water Deprivation Endocrine and Autonomic Systems Nucleic Acid Hybridization Rats Inbred Strains Rats medicine.anatomical_structure Hypothalamus Autoradiography Magnocellular cell Supraoptic Nucleus Nucleus hormones hormone substitutes and hormone antagonists Paraventricular Hypothalamic Nucleus |
| Zdroj: | Neuroendocrinology. 54:236-247 |
| ISSN: | 1423-0194 0028-3835 |
| DOI: | 10.1159/000125881 |
| Popis: | Vasopressin neuroendocrine function involves the regulation of both secretion and synthesis from magnocellular neuroendocrine cells but the coordination of these two processes is poorly understood. To explore the temporal relationship between physiological stimulation and vasopressin mRNA levels we measured vasopressin mRNA content within individual magnocellular neurons of the supraoptic and paraventricular nucleus during the course of water deprivation. Analysis of autoradiographic silver grain densities from in situ hybridization of an [125I]dCTP-labeled oligonucleotide specific for vasopressin mRNA revealed a wide variety of resting vasopressin mRNA levels and differential responses to water deprivation in the magnocellular neuroendocrine cells. During water deprivation, the vasopressin mRNA content of the paraventricular nucleus increases rapidly and with shorter latency and greater incremental response than the supraoptic nucleus. Double-labeling experiments with combined in situ hybridization and immunocytochemistry identified a population of vasopressin immunoreactive cells which maintain very low basal levels of vasopressin mRNA. The location of these cells correlates with the location of increased silver grain densities during water deprivation. One subset of vasopressin magnocellular neurons failed to show high levels of vasopressin mRNA, indicating that all cells are not equally responsive to water deprivation. These patterns of vasopressin mRNA expression suggest the presence of functional subpopulations of vasopressin neuroendocrine cells which may reflect stimulus-specific patterns of afferent input to the supraoptic and paraventricular nucleus. |
| Databáze: | OpenAIRE |
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