Quantitative analysis of four rapid antigen assays for detection of pandemic H1N1 2009 compared with seasonal H1N1 and H3N2 influenza A viruses on nasopharyngeal aspirates from patients with influenza
| Autor: | Y.P. Lam, Benjamin J. Cowling, Leo L.M. Poon, Y.L. Ho, K.M. Chan, H.L. Tong, Kwok-Hung Chan, Joseph S. M. Peiris |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Male Adolescent viruses Biology medicine.disease_cause Sensitivity and Specificity Virus Young Adult Influenza A Virus H1N1 Subtype Antigen Antigen assays Nasopharynx Virology Pandemic Influenza Human Influenza A virus medicine Humans In patient Child Antigens Viral Aged Aged 80 and over Immunoassay Clinical Laboratory Techniques Influenza A Virus H3N2 Subtype virus diseases Infant Influenza a Middle Aged respiratory tract diseases Child Preschool Immunology Female Viral load |
| Zdroj: | Journal of virological methods. 186(1-2) |
| ISSN: | 1879-0984 |
| Popis: | Data on analytical sensitivity of rapid diagnostic assays are important for clinical management of influenza, especially during a pandemic. Four rapid antigen detection assays were compared for detection of pandemic influenza A H1N1 2009, seasonal H1N1 and H3N2 in 96 patients with influenza A infection confirmed by real-time RT-PCR. These rapid antigen tests appeared to have lower sensitivity (55.8%) for the diagnosis of pandemic influenza A H1N1 2009 as compared with seasonal H3N2 (71.0%) or H1N1 (69.4%) influenza infections, a difference that was related to a lower viral load in patients infected with the pandemic influenza A H1N1 2009 virus. The detection limit of these antigen detection tests in clinical specimens was an influenza A M gene copy number of average 1.0×10(7) copies/ml. Significant variations between tests in sensitivity for detection of pandemic influenza A H1N1 2009 (43.4-63.3%) were observed. The Directigen EZ Influenza A+B and the Espline Influenza A+B had comparable sensitivity (63%) and were the most sensitive among the four assays evaluated. |
| Databáze: | OpenAIRE |
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