Circulating microRNA as biomarkers of canine mammary carcinoma in dogs
| Autor: | Annette N. Smith, Esther Gisela Martinez‐Romero, Richard C. Bird, Jey W. Koehler, Eric J. Fish, Patricia DeInnocentes, Nripesh Prasad |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Pathology
medicine.medical_specialty Lymphovascular invasion serum biomarkers canine Mammary Neoplasms Animal Standard Article Metastasis Dogs microRNA medicine Biomarkers Tumor Animals Circulating MicroRNA Dog Diseases Retrospective Studies mammary carcinoma Canine Mammary Carcinoma Messenger RNA lcsh:Veterinary medicine General Veterinary business.industry Sequence Analysis RNA Carcinoma Area under the curve Retrospective cohort study medicine.disease Standard Articles Oncology Lymphatic Metastasis lcsh:SF600-1100 Female SMALL ANIMAL business |
| Zdroj: | Journal of Veterinary Internal Medicine, Vol 34, Iss 3, Pp 1282-1290 (2020) Journal of Veterinary Internal Medicine |
| ISSN: | 0891-6640 1939-1676 |
| Popis: | Background Differentiating benign from canine malignant mammary tumors requires invasive surgical biopsy. Circulating microRNAs (miRNA) may represent promising minimally invasive cancer biomarkers in people and animals. Objectives To evaluate the serum mRNA profile between dogs with and without mammary carcinoma, and to determine if any of these markers have prognostic significance. Animals Ten healthy client‐owned female dogs (5 intact, 5 spayed) and 10 dogs with histologically confirmed mammary carcinoma were included; 9 were client‐owned, whereas 1 was a research colony dog. Methods Retrospective study. Serum miRNA was evaluated by RNA deep‐sequencing (RNAseq) and digital droplet PCR (dPCR).Expression of candidate biomarkers miR‐18a, miR‐19b, miR‐29b, miR‐34c, miR‐122, miR‐125a, and miR‐181a was compared with clinical characteristics, including grade, metastasis, and survival. Results 452 unique serum miRNAs were detected by RNAseq. Sixty‐five individual miRNAs were differentially expressed (>±1.5‐fold) and statistically significant between groups. Serum miR‐19b (P = .003) and miR‐125a (P |
| Databáze: | OpenAIRE |
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