Additional file 3 of A detached leaf assay for testing transient gene expression and gene editing in cowpea (Vigna unguiculata [L.] Walp.)

Autor: Juranić, Martina, Dilrukshi S. K. Nagahatenna, Rigel Salinas-Gamboa, Hand, Melanie L., Sánchez-León, Nidia, Weng Herng Leong, How, Tracy, Bazanova, Natalia, Spriggs, Andrew, Jean-Philippe Vielle-Calzada, Koltunow, Anna M. G.
Rok vydání: 2020
Předmět:
DOI: 10.6084/m9.figshare.12487148
Popis: Additional file 3: Fig. S3. Splice variants of the OSD1, REC8 and SPO11-1 genes identified in ovules at different developmental stages in cowpea. Refer to Additional File 6: Table S5 for more details. a-c Exons are shown as grey rectangles, while introns are represented by black lines. Different splice variants are shown underneath the exon-intron organization of a OSD1, b REC8 and c SPO11-1. d Semi-quantitative RT-PCR analysis of OSD1, REC8 and SPO11-1 mRNA. 1 µl of each cDNA was used for the PCR reaction. In the case of SPO11-1, a smear is visible representing a mixture of splicing variants. e Summary of results indicating the number of clones analyzed for each of three genes and the corresponding number of splice forms identified in reproductive tissues. Legend: fTET, female meiotic tetrads; MES, mature embryo sacs; MMC, differentiated megaspore mother cells.
Databáze: OpenAIRE