Identification of potential key genes and key pathways related to clear cell renal cell carcinoma through bioinformatics analysis
| Autor: | Wenxin Zhai, Shenghua Dong, Zhuang Yu, Jing Fang, Haijiao Lu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
Hydroxyacid oxidase 2 Biophysics Biology Biochemistry Pathogenesis 03 medical and health sciences 0302 clinical medicine Cell Line Tumor Gene expression medicine Biomarkers Tumor Humans Gene Regulatory Networks Protein Interaction Maps Formimidoyltransferase cyclodeaminase Gene Carcinoma Renal Cell 030304 developmental biology 0303 health sciences General Medicine medicine.disease Kidney Neoplasms Solute carrier family Neoplasm Proteins Gene Expression Regulation Neoplastic Clear cell renal cell carcinoma 030220 oncology & carcinogenesis Cancer research Biomarker (medicine) Female |
| Zdroj: | Acta biochimica et biophysica Sinica. 52(8) |
| ISSN: | 1745-7270 |
| Popis: | Clear cell renal cell carcinoma (ccRCC) is a common malignancy of the genitourinary system and is associated with high mortality rates. However, the molecular mechanism of ccRCC pathogenesis is still unclear, which translates to few effective diagnostic and prognostic biomarkers. In this study, we conducted a bioinformatics analysis on three Gene Expression Omnibus datasets and identified 437 differentially expressed genes (DEGs) related to ccRCC development and prognosis, of which 311 and 126 genes are respectively down-regulated and up-regulated. The protein-protein interaction network of these DEGs consists of 395 nodes and 1872 interactions and 2 prominent modules. The Staphylococcus aureus infection and complement and coagulation cascades are significantly enriched in module 1 and are likely involved in ccRCC progression. Forty-two hub genes were screened, of which von Willebrand factor, TIMP metallopeptidase inhibitor 1, plasminogen, formimidoyltransferase cyclodeaminase, solute carrier family 34 member 1, hydroxyacid oxidase 2, alanine-glyoxylate aminotransferase 2, phosphoenolpyruvate carboxykinase 1, and 3-hydroxy-3-methylglutaryl-CoA synthase 2 are possibly related to the prognosis of ccRCC. The differential expression of all nine genes was confirmed by quantitative real-time polymerase chain reaction analysis of the ccRCC and normal renal tissues. These key genes are potential biomarkers for the diagnosis and prognosis of ccRCC and warrant further investigation. |
| Databáze: | OpenAIRE |
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