The involvement of Gab1 and PI 3-kinase in β1 integrin signaling in keratinocytes
Autor: | Kunihiko Tamaki, Hiroko Nakashima, Mayumi Komine, Rei Watanabe, Yoshihiro Kuwano, Nobuko Ishiura, Hitoshi Okochi, Manabu Fujimoto, Tatsuo S. Hamazaki |
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Jazyk: | angličtina |
Rok vydání: | 2007 |
Předmět: |
Keratinocytes
MAPK/ERK pathway MAP Kinase Signaling System Biophysics Phosphatidylinositol 3-Kinases Biology HaCaT cell Biochemistry Cell Line chemistry.chemical_compound medicine Humans Phosphatidylinositol β1 Integrin Extracellular Signal-Regulated MAP Kinases Molecular Biology Adaptor Proteins Signal Transducing Phosphoinositide-3 Kinase Inhibitors Gab1 Stem cell Cell growth Integrin beta1 Cell Biology Mitogen-activated protein kinase Cell biology HaCaT medicine.anatomical_structure chemistry Cell culture Cancer research Keratinocyte Phosphatidylinositol 3-kinase |
Zdroj: | Biochemical and Biophysical Research Communications. 361(1):224-229 |
ISSN: | 0006-291X |
Popis: | 金沢大学大学院医学系研究科血管分子科学 The control of the stem cell compartment in epidermis is closely linked to the regulation of keratinocyte proliferation and differentiation. β1 integrins are expressed 2-fold higher by stem cells than transit-amplifying cells. Signaling from these β1 integrins is critical for the regulation of the epidermal stem cell compartment. To clarify the functional relevance of this differential expression of β1 integrins, we established HaCaT cells with high β1integrin expression by repeated flow cytometric sorting of this population from the parental cell line. In these obtained cells expressing β1 integrins by 5-fold, MAPK activation was markedly increased. Regarding the upstream of MAPK, Gab1 phosphorylation was also higher with high β1 integrin expression, while Shc phosphorylation was not altered. In addition, enhanced phosphatidylinositol 3-kinase activation was also observed. These observations suggest that Gab1 and phosphatidylinositol 3-kinase play pivotal roles in the β1 integrin-mediated regulation of the epidermal stem cell compartment. © 2007 Elsevier Inc. All rights reserved. |
Databáze: | OpenAIRE |
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