First report of Streptococcus agalactiae isolated from Oreochromis niloticus in Piura, Peru: Molecular identification and histopathological lesions
| Autor: | Alberto Manchego Sayán, Wilson Gómez Manrique, Mayra Araguaia Pereira Figueiredo, Nieves Nancy Sandoval Chaupe, Frank Barreiro Sánchez, Karina Huancaré Pusari, Yessica Ortega Asencios, Henry Ostos Alfonso, Hamilton Bueno Mendizábal, Marco Antonio de Andrade Belo |
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| Přispěvatelé: | Natl Univ San Marcos, Surcolombiana Univ, Universidade de São Paulo (USP), Univ Camilo Castelo Branco, Universidade Estadual Paulista (Unesp) |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty food.ingredient Fish farming Aquatic Science medicine.disease_cause lcsh:Aquaculture. Fisheries. Angling law.invention Microbiology 03 medical and health sciences Nile tilapia food law medicine Pathogen Polymerase chain reaction Inflammation lcsh:SH1-691 biology Bacteria Tilapia 04 agricultural and veterinary sciences biology.organism_classification Oreochromis Tilapid 030104 developmental biology PCR Streptococcus agalactiae 040102 fisheries 0401 agriculture forestry and fisheries Animal Science and Zoology Histopathology human activities |
| Zdroj: | Aquaculture Reports, Vol 4, Iss C, Pp 74-79 (2016) Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 2352-5134 |
| DOI: | 10.1016/j.aqrep.2016.06.002 |
| Popis: | Made available in DSpace on 2019-10-04T18:34:45Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-11-01 Vice Rectorate of Research - High Council of Research - of the San Marcos University The aim of this study was to identify the bacterium Streptococcus agalactiae isolated in farmed Nile tilapia (Oreochromis niloticus) from Piura, Peru and to characterize the histopathological lesions caused by this pathogen. Sixteen tilapias were sampled with clinic signs of the disease such as erratic swimming, exophthalmia and haemorrhages on the body and fins. Qualitative PCR in real time and histopathological analysis were performed. Nine fishes positives to S. agalactiae were found. The main histopathological findings were fibrinosuppurative epicarditis, periesplenitis, meninigitis and panophtaltmitis with predominance of mononuclear infiltration in all tissues. The correlation between qPCR and histopathological findings demonstrated nine fish (prevalence of 56.25%) with Cq lower than 30, associated to high degree of tissue injuries. This study reports the first isolation of S. agalactiae by PCR in real time in tilapia farmed in Peru and characterizes the major histopathological changes caused by this bacterium. (C) 2016 The Authors. Published by Elsevier B.V. Natl Univ San Marcos, Sch Vet Med, Dept Ictiopathol, Av Circunvalac 2800, Lima 2800, Peru Surcolombiana Univ, Lab Genom Med, St 9 14-03, Barrio Altico, Neiva Huila, Colombia Univ Sao Paulo, Inst Biomed Sci, Dept Parasitol, Av Prof Lineu Prestes 1374, BR-05508900 Sao Paulo, SP, Brazil Univ Camilo Castelo Branco, Lab Vet Pathol, Av Hilario da Silva Passos 950,Parque Univ, BR-13690000 Descalvado, SP, Brazil Sao Paulo State Univ, Via Acesso Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, Brazil Sao Paulo State Univ, Via Acesso Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, Brazil Vice Rectorate of Research - High Council of Research - of the San Marcos University: 140 801 031 |
| Databáze: | OpenAIRE |
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